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学科主题: 物理化学
题名: Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry
作者: Wang, Tingting1, 2;  Fekete, Agnes3;  Gaspar, Andras3;  Ma, Junfeng1;  Liang, Zhen1;  Yuan, Huiming1;  Zhang, Lihua1;  Schmitt-Kopplin, Philippe3;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Capillary isoelectric focusing ;  Low molecular weight acids ;  Mass spectrometry ;  Monolithic immobilized pH gradient
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2011-02-01
DOI: 10.1002/jssc.201000720
卷: 34, 期:4, 页:422-427
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry
研究领域[WOS]: Chemistry
英文摘要: A novel method for the separation and detection of low molecular weight (LMW) acids was developed using monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry. Two main parameters, focusing conditions and delivery buffer conditions, which might affect separation efficiency, were optimized with the focusing time of 7 min at 350 V/cm and the delivery buffer of 50% (v/v) acetonitrile in 10 mmol/L ammonium formate (pH 3.0). Under these conditions, the linear correlation between the volume of delivery solvent and the pK(a) of the model components was observed. In addition, the separation mechanism of LMW acids was proposed as well. We suppose that this method may provide a useful tool for the characterization of LMW components (e.g. natural organic matter of different origins).
关键词[WOS]: PHASE LIQUID-CHROMATOGRAPHY ;  ZONE-ELECTROPHORESIS ;  PROTEIN-ANALYSIS ;  ONLINE ;  MARKERS ;  POINTS ;  DEVICE ;  COLUMN
语种: 英语
WOS记录号: WOS:000287942000008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115316
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
3.German Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Inst Ecol Chem, Neuherberg, Germany

Recommended Citation:
Wang, Tingting,Fekete, Agnes,Gaspar, Andras,et al. Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(4):422-427.
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