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学科主题: 物理化学
题名: A novel organic-inorganic hybrid monolith for trypsin immobilization
作者: Wu ShuaiBin1, 2;  Ma JunFeng1, 2;  Yang KaiGuang1;  Liu JinXiang1, 2;  Liang Zhen1;  Zhang LiHua1;  Zhang YuKui1
通讯作者: 张丽华
关键词: immobilized enzyme reactor ;  organic-inorganic hybrid monolith ;  protein identification ;  HPLC-MS/MS
刊名: SCIENCE CHINA-LIFE SCIENCES
发表日期: 2011
DOI: 10.1007/s11427-010-4108-z
卷: 54, 期:1, 页:54-59
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biology
摘要: A novel organic-inorganic hybrid monolith for trypsin immobilization
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
英文摘要: In proteomics, attention has focused on various immobilized enzyme reactors (IMERs) for the realization of high throughput digestion. In this report, a novel organic-inorganic hybrid monolith based IMER was prepared in a 100 mu m i.d. capillary with 3-glycidoxypropyltrimethoxysilane (GLYMO) as the monomer and tetraethoxysilane (TEOS) as the crosslinker. Trypsin immobilization was achieved via the reaction between vicinal diol groups, which were obtained from hydrolysis of epoxy groups, and the amino groups of trypsin. Bovine serum albumin was digested thoroughly by this IMER in 47 s. After micro-reverse phase liquid chromatography-tandem mass spectrometry (mu RPLC-MS/MS) analysis and database searching, beyond 35% sequence coverage was obtained, and the result was comparable to that of 12 h in solution digestion. The present IMER has potential for high throughput digestion.
关键词[WOS]: PROTEIN DIGESTION ;  IDENTIFICATION ;  SYSTEM ;  PROTEOLYSIS ;  REACTOR ;  ONLINE
语种: 英语
WOS记录号: WOS:000286470400008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115318
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Wu ShuaiBin,Ma JunFeng,Yang KaiGuang,et al. A novel organic-inorganic hybrid monolith for trypsin immobilization[J]. SCIENCE CHINA-LIFE SCIENCES,2011,54(1):54-59.
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