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学科主题: 物理化学
题名: Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for proteome research
作者: Tao, Dingyin1, 2;  Zhang, Lihua1;  Shan, Yichu1;  Liang, Zhen1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Proteome ;  Micro-HPLC ;  Nano-HPLC ;  Multi-dimensional separation ;  MS-MS
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2011
DOI: 10.1007/s00216-010-3946-7
卷: 399, 期:1, 页:229-241
收录类别: SCI
文章类型: Review
部门归属: 18
项目归属: 1810
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for proteome research
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: High-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS-MS) is regarded as one of the most powerful techniques for separation and identification of proteins. Recently, much effort has been made to improve the separation capacity, detection sensitivity, and analysis throughput of micro- and nano-HPLC, by increasing column length, reducing column internal diameter, and using integrated techniques. Development of HPLC columns has also been rapid, as a result of the use of submicrometer packing materials and monolithic columns. All these innovations result in clearly improved performance of micro- and nano-HPLC for proteome research.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  MALDI-TOF-MS ;  SIZE-EXCLUSION CHROMATOGRAPHY ;  HUMAN LIVER-TISSUE ;  REVERSED-PHASE ;  MONOLITHIC COLUMNS ;  ELECTROSPRAY-IONIZATION ;  SHOTGUN PROTEOMICS ;  ESI-MS/MS ;  MULTIDIMENSIONAL SEPARATIONS
语种: 英语
WOS记录号: WOS:000285781200024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115319
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Tao, Dingyin,Zhang, Lihua,Shan, Yichu,et al. Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for proteome research[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(1):229-241.
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