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学科主题: 物理化学
题名: Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins
作者: Gai, Qingqing1;  Qu, Feng1;  Zhang, Tao1;  Zhang, Yukui2
通讯作者: 屈锋 ;  张玉奎
关键词: Carboxyl modified magnetic particles ;  Aqueous two-phase extraction ;  Protein ;  Magnetic separation
刊名: TALANTA
发表日期: 2011-07-15
DOI: 10.1016/j.talanta.2011.03.055
卷: 85, 期:1, 页:304-309
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2,4
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins
研究领域[WOS]: Chemistry
英文摘要: Both of the magnetic particle adsorption and aqueous two-phase extraction (ATPE) were simple, fast and low-cost method for protein separation. Selective proteins adsorption by carboxyl modified magnetic particles was investigated according to protein isoelectric point, solution pH and ionic strength. Aqueous two-phase system of PEG/sulphate exhibited selective separation and extraction for proteins before and after magnetic adsorption. The two combination ways, magnetic adsorption followed by ATPE and ATPE followed by magnetic adsorption, for the separation of proteins mixture of lysozyme, bovine serum albumin, trypsin, cytochrome C and myloglobin were discussed and compared. The way of magnetic adsorption followed by ATPE was also applied to human serum separation. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: PROTEOMIC ANALYSIS ;  SYSTEMS ;  PURIFICATION ;  COPOLYMER ;  LYSOZYME ;  SERUM ;  BEADS
语种: 英语
WOS记录号: WOS:000292848900041
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115324
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Gai, Qingqing,Qu, Feng,Zhang, Tao,et al. Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins[J]. TALANTA,2011,85(1):304-309.
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