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学科主题物理化学
Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins
Gai, Qingqing1; Qu, Feng1; Zhang, Tao1; Zhang, Yukui2; Qu F(屈锋); Zhang YK(张玉奎)
关键词Carboxyl Modified Magnetic Particles Aqueous Two-phase Extraction Protein Magnetic Separation
刊名TALANTA
2011-07-15
ISSN待补充
DOI10.1016/j.talanta.2011.03.055
85期:1页:304-309
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名2,4
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PROTEOMIC ANALYSIS ; SYSTEMS ; PURIFICATION ; COPOLYMER ; LYSOZYME ; SERUM ; BEADS
英文摘要Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins; Both of the magnetic particle adsorption and aqueous two-phase extraction (ATPE) were simple, fast and low-cost method for protein separation. Selective proteins adsorption by carboxyl modified magnetic particles was investigated according to protein isoelectric point, solution pH and ionic strength. Aqueous two-phase system of PEG/sulphate exhibited selective separation and extraction for proteins before and after magnetic adsorption. The two combination ways, magnetic adsorption followed by ATPE and ATPE followed by magnetic adsorption, for the separation of proteins mixture of lysozyme, bovine serum albumin, trypsin, cytochrome C and myloglobin were discussed and compared. The way of magnetic adsorption followed by ATPE was also applied to human serum separation. (C) 2011 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000292848900041
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115324
专题中国科学院大连化学物理研究所
通讯作者Qu F(屈锋); Zhang YK(张玉奎)
作者单位1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式
GB/T 7714
Gai, Qingqing,Qu, Feng,Zhang, Tao,et al. Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins[J]. TALANTA,2011,85(1):304-309.
APA Gai, Qingqing,Qu, Feng,Zhang, Tao,Zhang, Yukui,屈锋,&张玉奎.(2011).Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins.TALANTA,85(1),304-309.
MLA Gai, Qingqing,et al."Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins".TALANTA 85.1(2011):304-309.
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