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学科主题: 物理化学
题名: The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation
作者: Gai, Qing-Qing1;  Qu, Feng1;  Zhang, Tao1;  Zhang, Yu-Kui2
通讯作者: 屈锋 ;  张玉奎
关键词: Molecularly imprinted polymer ;  Magnetic particles ;  Bovine serum albumin ;  Protein separation
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2011-06-03
DOI: 10.1016/j.chroma.2011.03.069
卷: 1218, 期:22, 页:3489-3495
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2,4
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Currently, small proteins imprinting are more reported since large proteins molecular imprinting faces challenge due to their bulk size and complex structure. In this work, bovine serum albumin (BSA) surface-imprinted magnetic polymer was successfully synthesized based on atomic transfer radical polymerization (ATRP) method in the presence of common monomer (N-isopropylacrylamide) with the assistant of basic functional monomer (N-[3-(dimethylamino)propyl]-methacrylamide), which provides a achievable attempt for imprinting larger target proteins based on the ATPR with the mild reaction conditions. The BSA-imprinted polymer exhibited higher adsorption capacity and selectivity to BSA over the non-imprinted polymer. Competitive adsorption tests indicated the BSA-imprinted polymer had better selective adsorption and recognition properties to BSA in the mixture. The obtained BSA-imprinted polymer was applied to bovine serum, which also showed selectivity to BSA. In addition, a conventional aqueous two-phase solution of PEG/sulphate was used as elution for adsorbed BSA, which was compared with common NaCl elution. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: SUBMICROMETER PARTICLES ;  MAGNETIC-SUSCEPTIBILITY ;  RECOGNITION PROPERTY ;  AQUEOUS-MEDIA ;  HYDROGEL ;  MICROSPHERES ;  LYSOZYME ;  BEADS
语种: 英语
WOS记录号: WOS:000291132600011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115325
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Gai, Qing-Qing,Qu, Feng,Zhang, Tao,et al. The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(22):3489-3495.
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