DICP OpenIR
Subject Area物理化学
Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins
Qin, Lei1; He, Xi-Wen1; Yuan, Xia1; Li, Wen-You1; Zhang, Yu-Kui1,2; Li WY(李文友); Zhang YK(张玉奎)
KeywordThermosensitive Molecularly Imprinted Bead Protein
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2011-04-01
ISSN待补充
DOI10.1007/s00216-011-4736-6
Volume399Issue:10Pages:3375-3385
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank2,5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordBOVINE SERUM-ALBUMIN ; INIFERTER TECHNIQUE ; POLYMERS ; ADSORPTION ; HYDROGELS ; GELS ; SEPARATION
AbstractMolecularly imprinted beads with double thermosensitive gates for selective recognition of proteins; A new approach is reported on the use of poly(N-isopropylacrylamide) (PNIPAM)-coated molecularly imprinted beads (coated MIP beads) for controlling the release of protein. The coated MIP beads were composed of double layers, an internal thermosensitive lysozyme-imprinted layer, and an external PNIPAM layer. The coated MIP beads were prepared by two-step surface-initiated living-radical polymerization (SIP). In this systemic study, the coated MIP beads had good selectivity to the template protein (lysozyme) and temperature stimulus-responsive behavior, both of which were superior to those of MIP beads having a layer of thermosensitive lysozyme-imprinted polymer only. Using the coated MIP beads, reference proteins and the template lysozyme could be released separately at 38 A degrees C and at 23 A degrees C. The corresponding coated non-imprinted beads (coated NIP beads) did not have such double thermosensitive "gates" with specific selectivity for a particular protein. The proposed smart controlled imprinted system for protein is attractive for chemical carriers, drug-delivery system, and sensors.
Language英语
WOS IDWOS:000288398700007
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115327
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi WY(李文友); Zhang YK(张玉奎)
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Qin, Lei,He, Xi-Wen,Yuan, Xia,et al. Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(10):3375-3385.
APA Qin, Lei.,He, Xi-Wen.,Yuan, Xia.,Li, Wen-You.,Zhang, Yu-Kui.,...&张玉奎.(2011).Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,399(10),3375-3385.
MLA Qin, Lei,et al."Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 399.10(2011):3375-3385.
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