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学科主题: 物理化学
题名: Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins
作者: Qin, Lei1;  He, Xi-Wen1;  Yuan, Xia1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2
通讯作者: 李文友 ;  张玉奎
关键词: Thermosensitive ;  Molecularly imprinted bead ;  Protein
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2011-04-01
DOI: 10.1007/s00216-011-4736-6
卷: 399, 期:10, 页:3375-3385
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2,5
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A new approach is reported on the use of poly(N-isopropylacrylamide) (PNIPAM)-coated molecularly imprinted beads (coated MIP beads) for controlling the release of protein. The coated MIP beads were composed of double layers, an internal thermosensitive lysozyme-imprinted layer, and an external PNIPAM layer. The coated MIP beads were prepared by two-step surface-initiated living-radical polymerization (SIP). In this systemic study, the coated MIP beads had good selectivity to the template protein (lysozyme) and temperature stimulus-responsive behavior, both of which were superior to those of MIP beads having a layer of thermosensitive lysozyme-imprinted polymer only. Using the coated MIP beads, reference proteins and the template lysozyme could be released separately at 38 A degrees C and at 23 A degrees C. The corresponding coated non-imprinted beads (coated NIP beads) did not have such double thermosensitive "gates" with specific selectivity for a particular protein. The proposed smart controlled imprinted system for protein is attractive for chemical carriers, drug-delivery system, and sensors.
关键词[WOS]: BOVINE SERUM-ALBUMIN ;  INIFERTER TECHNIQUE ;  POLYMERS ;  ADSORPTION ;  HYDROGELS ;  GELS ;  SEPARATION
语种: 英语
WOS记录号: WOS:000288398700007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115327
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Qin, Lei,He, Xi-Wen,Yuan, Xia,et al. Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(10):3375-3385.
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