DICP OpenIR
学科主题物理化学
A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein
Qin, Lei1; He, Xi-Wen1; Jia, Man1; Li, Wen-You1; Zhang, Yu-Kui1,2; Zhang YK(张玉奎)
关键词Antigens Imprinting Monolith Columns Proteins Thermosensitivity
刊名CHEMISTRY-A EUROPEAN JOURNAL
2011
ISSN待补充
DOI10.1002/chem.201000875
17期:5页:1696-1704
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名2,5
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]MOLECULARLY IMPRINTED POLYMERS ; LIQUID-CHROMATOGRAPHY ; RESPONSIVE POLYMER ; RECOGNITION ; GELS ; ADSORPTION ; SILICA ; TEMPERATURE ; HEMOGLOBIN ; HYDROGELS
英文摘要A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein; The main objective of this study was to develop a new methodology for the preparation of a protein (antigen) that is a molecularly imprinted polymer (MIP, an artificial antibody) modified onto the surface of a silica skeleton in which the resulting stationary phase is thermosensitive. The silica monolithic skeleton with vinyl groups was synthesized in a stainless-steel column by using a mild one-step sol gel process with two types of precursor: methyltrimethoxysilane (MTMS) and gamma-methacryloxypropyltrimethoxysilane (gamma-MAPS). Subsequently, three types of the thermosensitive protein MIP were anchored onto the surface of the silica skeleton to prepare the MIP monoliths, which were systematically investigated for back pressure and separation ability at different temperatures to establish good imprinting conditions. Under the optimized imprinting conditions, the chromatographic behavior of the thermosensitive MIP monolith exhibited strong retention ability for the lysozyme template (target antigen) in relation to the non-imprinting monolith (NIP monolith). The imprinting factor (IF) for lysozyme reached 3.48 at 20 C. Moreover, this new type of artificial antibody displayed favorable binding characteristics for lysozyme over competitive proteins and was further evaluated to selectively separate lysozyme in a real sample by using an on-line method. The run-to-run and column-to-column repeatability measurements of the thermosensitive MIP monoliths were also satisfactory.
语种英语
WOS记录号WOS:000287555600035
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115331
专题中国科学院大连化学物理研究所
通讯作者Zhang YK(张玉奎)
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Qin, Lei,He, Xi-Wen,Jia, Man,et al. A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein[J]. CHEMISTRY-A EUROPEAN JOURNAL,2011,17(5):1696-1704.
APA Qin, Lei,He, Xi-Wen,Jia, Man,Li, Wen-You,Zhang, Yu-Kui,&张玉奎.(2011).A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein.CHEMISTRY-A EUROPEAN JOURNAL,17(5),1696-1704.
MLA Qin, Lei,et al."A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein".CHEMISTRY-A EUROPEAN JOURNAL 17.5(2011):1696-1704.
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