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学科主题: 物理化学
题名: A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein
作者: Qin, Lei1;  He, Xi-Wen1;  Jia, Man1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2
通讯作者: 张玉奎
关键词: antigens ;  imprinting ;  monolith columns ;  proteins ;  thermosensitivity
刊名: CHEMISTRY-A EUROPEAN JOURNAL
发表日期: 2011
DOI: 10.1002/chem.201000875
卷: 17, 期:5, 页:1696-1704
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2,5
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
摘要: A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein
研究领域[WOS]: Chemistry
英文摘要: The main objective of this study was to develop a new methodology for the preparation of a protein (antigen) that is a molecularly imprinted polymer (MIP, an artificial antibody) modified onto the surface of a silica skeleton in which the resulting stationary phase is thermosensitive. The silica monolithic skeleton with vinyl groups was synthesized in a stainless-steel column by using a mild one-step sol gel process with two types of precursor: methyltrimethoxysilane (MTMS) and gamma-methacryloxypropyltrimethoxysilane (gamma-MAPS). Subsequently, three types of the thermosensitive protein MIP were anchored onto the surface of the silica skeleton to prepare the MIP monoliths, which were systematically investigated for back pressure and separation ability at different temperatures to establish good imprinting conditions. Under the optimized imprinting conditions, the chromatographic behavior of the thermosensitive MIP monolith exhibited strong retention ability for the lysozyme template (target antigen) in relation to the non-imprinting monolith (NIP monolith). The imprinting factor (IF) for lysozyme reached 3.48 at 20 C. Moreover, this new type of artificial antibody displayed favorable binding characteristics for lysozyme over competitive proteins and was further evaluated to selectively separate lysozyme in a real sample by using an on-line method. The run-to-run and column-to-column repeatability measurements of the thermosensitive MIP monoliths were also satisfactory.
关键词[WOS]: MOLECULARLY IMPRINTED POLYMERS ;  LIQUID-CHROMATOGRAPHY ;  RESPONSIVE POLYMER ;  RECOGNITION ;  GELS ;  ADSORPTION ;  SILICA ;  TEMPERATURE ;  HEMOGLOBIN ;  HYDROGELS
语种: 英语
WOS记录号: WOS:000287555600035
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115331
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Qin, Lei,He, Xi-Wen,Jia, Man,et al. A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein[J]. CHEMISTRY-A EUROPEAN JOURNAL,2011,17(5):1696-1704.
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