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学科主题: 物理化学
题名: Metabolite profiling of Arabidopsis seedlings in response to exogenous sinalbin and sulfur deficiency
作者: Zhang, Jixiu1;  Sun, Xiumei1;  Zhang, Zhiping1;  Ni, Yuwen1;  Zhang, Qing1;  Liang, Xinmiao1;  Xiao, Hongbin1;  Chen, Jiping1;  Tokuhisa, James G.2
通讯作者: 陈吉平
关键词: Arabidopsis thaliana ;  Cruciferae ;  GC-TOF-MS ;  LC-MS ;  Metabolite profiling ;  Sinalbin ;  Sulfur
刊名: PHYTOCHEMISTRY
发表日期: 2011-10-01
DOI: 10.1016/j.phytochem.2011.06.002
卷: 72, 期:14-15, 页:1767-1778
收录类别: SCI
文章类型: Article
部门归属: 1;18
项目归属: 103;1803;1804
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Plant Sciences
摘要: Metabolite profiling of Arabidopsis seedlings in response to exogenous sinalbin and sulfur deficiency
研究领域[WOS]: Biochemistry & Molecular Biology ;  Plant Sciences
英文摘要: In order to determine how plant uptake of a sulfur-rich secondary metabolite, sinalbin, affects the metabolic profile of sulfur-deficient plants, gas chromatography time-of-flight mass spectrometry (GC-TOF-MS), in combination with liquid chromatography-mass spectrometry (LC-MS), was used to survey the metabolome of Arabidopsis seedlings grown in nutrient media under different sulfur conditions. The growth media had either sufficient inorganic sulfur for normal plant growth or insufficient inorganic sulfur in the presence or absence of supplementation with organic sulfur in the form of sinalbin (p-hydroxybenzylglucosinolate). A total of 90 metabolites were identified by GC-TOF-MS and their levels were compared across the three treatments. Of the identified compounds, 21 showed similar responses in plants that were either sulfur deficient or sinalbin supplemented compared to sulfur-sufficient plants, while 12 metabolites differed in abundance only in sulfur-deficient plants. Twelve metabolites accumulated to higher levels in sinalbin-supplemented than in the sulfur-sufficient plants. Secondary metabolites such as flavonol conjugates, sinapinic acid esters and glucosinolates, were identified by LC-MS and their corresponding mass fragmentation patterns were determined. Under sinalbin-supplemented conditions, sinalbin was taken up by Arabidopsis and contributed to the endogenous formation of glucosinolates. Additionally, levels of flavonol glycosides and sinapinic acid esters increased while levels of flavonol diglycosides with glucose attached to the 3-position were reduced. The exogenously administered sinalbin resulted in inhibition of root and hypocotyl growth and markedly influenced metabolite profiles, compared to control and sulfur-deficient plants. These results indicate that, under sulfur deficient conditions, glucosinolates can be a sulfur source for plants. This investigation defines an opportunity to elucidate the mechanism of glucosinolate degradation in vivo. (C) 2011 Elsevier Ltd. All rights reserved.
关键词[WOS]: CHROMATOGRAPHY MASS-SPECTROMETRY ;  GAS-CHROMATOGRAPHY ;  THALIANA ;  IDENTIFICATION ;  ACCUMULATION ;  PLANTS ;  MS ;  TRANSCRIPTOME ;  METABOLOMICS ;  SEPARATION
语种: 英语
WOS记录号: WOS:000294973300008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115333
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Virginia Tech, Dept Hort, Blacksburg, VA 24061 USA

Recommended Citation:
Zhang, Jixiu,Sun, Xiumei,Zhang, Zhiping,et al. Metabolite profiling of Arabidopsis seedlings in response to exogenous sinalbin and sulfur deficiency[J]. PHYTOCHEMISTRY,2011,72(14-15):1767-1778.
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