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学科主题: 物理化学
题名: Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS
作者: Cai, Xiaoming1;  Dong, Jun1;  Zou, Lijuan1, 2;  Xue, Xingya1;  Zhang, Xiuli1;  Liang, Xinmiao1
通讯作者: 张秀莉 ;  梁鑫淼
关键词: UPLC/Q-TOF ;  Hydrophilic interaction chromatography ;  Metabonomics ;  Lung cancer ;  Highly polar metabolites ;  Radiotherapy
刊名: CHROMATOGRAPHIA
发表日期: 2011-09-01
DOI: 10.1007/s10337-011-2077-4
卷: 74, 期:5-6, 页:391-398
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this study, plasma samples were collected from 28 health volunteers and 66 lung cancer patients. Ultraperformance HILIC/Q-TOF MS-based metabonomic techniques were employed to compare the highly polar metabolites (HPMs) in the plasma of patients with lung cancer and those in the plasma of healthy volunteers. High separation efficiency and good repeatability of the HILIC/Q-TOF MS method were observed. Partial least squares discriminant analysis (PLS-DA) of the data from both ESI+ and ESI- mode revealed good classifications between the health volunteer and the lung cancer patients. This result showed that the HPMs from lung cancer patients and healthy controls were significantly different. The correct classification rate of models in both ion modes reached 100%, indicating that the metabonomics method established in this study could predict the plasma samples with high accuracy. Besides, we found 19 ions that show a significant difference in levels between lung cancer patients and healthy controls. Finally, we conducted a primary study of the effect of radiotherapy on HPMs in lung cancer patients. Clear differences among the HPMs of patients in different radiotherapy periods and significant changes in levels of the 19 ions after radiotherapy were noted. This demonstrated that radiotherapy influences the metabolism system of patients.
关键词[WOS]: HYDROPHILIC-INTERACTION CHROMATOGRAPHY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  TANDEM MASS-SPECTROMETRY ;  RAT URINE ;  ZUCKER RAT ;  MULTIVARIATE-ANALYSIS ;  KIDNEY CANCER ;  LC-MS/MS ;  SERUM ;  SEPARATION
语种: 英语
WOS记录号: WOS:000294066000006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115334
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China
2.Dalian Med Univ, Hosp 2, Dalian 116023, Peoples R China

Recommended Citation:
Cai, Xiaoming,Dong, Jun,Zou, Lijuan,et al. Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS[J]. CHROMATOGRAPHIA,2011,74(5-6):391-398.
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