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学科主题: 物理化学
题名: Selective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid-phase dispersion using titania
作者: Xu, Lingyan1;  Shi, Hui1;  Liang, Tu1;  Feng, Jiatao2;  Jin, Yu1;  Ke, Yanxiong1;  Liang, Xinmiao1, 2
通讯作者: 柯燕雄
关键词: Dalbergia odorifera ;  Flavonoid glycosides ;  Matrix solid-phase dispersion ;  Selective separation ;  Titania
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2011-06-01
DOI: 10.1002/jssc.201100024
卷: 34, 期:11, 页:1347-1354
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 2,4
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Selective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid-phase dispersion using titania
研究领域[WOS]: Chemistry
英文摘要: Dalbergia odorifera contains high concentrations of flavonoid aglycones and trace flavonoid glycosides. In this study, trace flavonoid glycosides were separated from D. odorifera by titania with matrix solid-phase dispersion (MSPD). Before the MSPD experiment, four standards, including two isoflavone glycosides (genistin and formononetin-8-C-apiosyl (1-6)-glucoside) and their aglycones (genistein and formononetin), were used to compare their retention on a titania column. The effect of acetonitrile concentration and pH on their retention was investigated and a conclusion was drawn that high acetonitrile concentration and pH lead to the greatest difference in the retention of flavonoid as glycosides and aglycones. Besides hydrophilic interaction and ligand-exchange interaction may exist between sugar moiety of flavonoid glycoside and titania, so that flavonoid glycosides have stronger retention than that of aglycones. Based on the chromatographic rule of flavonoid as glycosides and aglycones on the titania column, the MSPD method was optimized to elute high concentration flavonoid aglycones first with 90% acetonitrile and 10% water containing 100 mM ammonium acetate buffer, and then to elute trace flavonoid glycosides with 20% acetonitrile and 80% water containing 1% trifluoroacetate (TFA). Isolated flavonoid glycosides were further analyzed by UPLC-MS/MS, and their fragmentation in MS 2 showed they are C-glycosyl flavonoids.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  IONIZATION MASS-SPECTROMETRY ;  ION CHROMATOGRAPHY ;  HPLC ;  PHOSPHOPEPTIDES ;  EXTRACTION ;  BEHAVIOR ;  COLUMN
语种: 英语
WOS记录号: WOS:000291346400017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115336
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian, Peoples R China

Recommended Citation:
Xu, Lingyan,Shi, Hui,Liang, Tu,et al. Selective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid-phase dispersion using titania[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(11):1347-1354.
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