DICP OpenIR
Subject Area物理化学
Selective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid-phase dispersion using titania
Xu, Lingyan1; Shi, Hui1; Liang, Tu1; Feng, Jiatao2; Jin, Yu1; Ke, Yanxiong1; Liang, Xinmiao1,2; Ke YX(柯燕雄)
KeywordDalbergia Odorifera Flavonoid Glycosides Matrix Solid-phase Dispersion Selective Separation Titania
Source PublicationJOURNAL OF SEPARATION SCIENCE
2011-06-01
ISSN待补充
DOI10.1002/jssc.201100024
Volume34Issue:11Pages:1347-1354
Indexed BySCI
SubtypeArticle
Department18
Funding Project1803
Contribution Rank2,4
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; IONIZATION MASS-SPECTROMETRY ; ION CHROMATOGRAPHY ; HPLC ; PHOSPHOPEPTIDES ; EXTRACTION ; BEHAVIOR ; COLUMN
AbstractSelective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid-phase dispersion using titania; Dalbergia odorifera contains high concentrations of flavonoid aglycones and trace flavonoid glycosides. In this study, trace flavonoid glycosides were separated from D. odorifera by titania with matrix solid-phase dispersion (MSPD). Before the MSPD experiment, four standards, including two isoflavone glycosides (genistin and formononetin-8-C-apiosyl (1-6)-glucoside) and their aglycones (genistein and formononetin), were used to compare their retention on a titania column. The effect of acetonitrile concentration and pH on their retention was investigated and a conclusion was drawn that high acetonitrile concentration and pH lead to the greatest difference in the retention of flavonoid as glycosides and aglycones. Besides hydrophilic interaction and ligand-exchange interaction may exist between sugar moiety of flavonoid glycoside and titania, so that flavonoid glycosides have stronger retention than that of aglycones. Based on the chromatographic rule of flavonoid as glycosides and aglycones on the titania column, the MSPD method was optimized to elute high concentration flavonoid aglycones first with 90% acetonitrile and 10% water containing 100 mM ammonium acetate buffer, and then to elute trace flavonoid glycosides with 20% acetonitrile and 80% water containing 1% trifluoroacetate (TFA). Isolated flavonoid glycosides were further analyzed by UPLC-MS/MS, and their fragmentation in MS 2 showed they are C-glycosyl flavonoids.
Language英语
WOS IDWOS:000291346400017
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115336
Collection中国科学院大连化学物理研究所
Corresponding AuthorKe YX(柯燕雄)
Affiliation1.E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Xu, Lingyan,Shi, Hui,Liang, Tu,et al. Selective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid-phase dispersion using titania[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(11):1347-1354.
APA Xu, Lingyan.,Shi, Hui.,Liang, Tu.,Feng, Jiatao.,Jin, Yu.,...&柯燕雄.(2011).Selective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid-phase dispersion using titania.JOURNAL OF SEPARATION SCIENCE,34(11),1347-1354.
MLA Xu, Lingyan,et al."Selective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid-phase dispersion using titania".JOURNAL OF SEPARATION SCIENCE 34.11(2011):1347-1354.
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