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学科主题: 物理化学
题名: Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction
作者: Li Cun-man2;  Xiao Yuan-sheng1;  Xue Xing-ya1;  Feng Jia-tao1;  Zhang Xiu-li1;  Liang Xin-miao1
通讯作者: 张秀莉 ;  梁鑫淼
关键词: Unstable compound ;  Isolation and purification ;  Preparative high performance liquid chromatography ;  Solid-phase extraction ;  Iridoid glucoside
刊名: CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
发表日期: 2011-05-01
卷: 27, 期:3, 页:392-396
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,2
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
摘要: Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction
研究领域[WOS]: Chemistry
英文摘要: An efficient preparative method was successfully developed for isolation and purification of unstable components from medicinal plant extracts, using a combined method of preparative high performance liquid chromatography(HPLC) and solid-phase extraction(SPE). The aim of this study was to obtain an effective method with high preparative efficiency and importantly to avoid the transformation of unstable compounds. The preparative HPLC system was based on an LC/MS controlled four-channel autopurification system. The SPE method was performed with a C(18) packing material to trap the target compounds and to remove the acidic additive derived from the mobile phase. Using this method, the unstable iridoid glucosides(IGs) as model compounds were successfully isolated and purified from the extract of Hedyotis diffusa Willd. Six IGs(including one new minor IG) and one nucleotide compound were simultaneously obtained, each with a purity of > 91% as determined by HPLC. The structures of the isolated compounds were identified by UPLC/Q-TOF MS, UV, 1D and/or 2D NMR. It was demonstrated that the combination of preparative HPLC with SPE is a versatile tool for preparative purification of unstable compounds from complex natural products.
关键词[WOS]: MASS-SPECTROMETRY ;  MONOTERPENE GLUCOSIDES ;  NATURAL-PRODUCTS ;  FRACTIONATION ;  IONIZATION ;  GLYCOSIDES ;  DIFFUSA
语种: 英语
WOS记录号: WOS:000291505100011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115343
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Hebei Univ Sci & Technol, Hebei Res Ctr Anal & Testing, Shijiazhuang 050018, Peoples R China

Recommended Citation:
Li Cun-man,Xiao Yuan-sheng,Xue Xing-ya,et al. Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2011,27(3):392-396.
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