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学科主题物理化学
Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction
Li Cun-man2; Xiao Yuan-sheng1; Xue Xing-ya1; Feng Jia-tao1; Zhang Xiu-li1; Liang Xin-miao1; Zhang XL(张秀莉); Liang XM(梁鑫淼)
关键词Unstable Compound Isolation And Purification Preparative High Performance Liquid Chromatography Solid-phase Extraction Iridoid Glucoside
刊名CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
2011-05-01
ISSN待补充
27期:3页:392-396
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名1,2
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]MASS-SPECTROMETRY ; MONOTERPENE GLUCOSIDES ; NATURAL-PRODUCTS ; FRACTIONATION ; IONIZATION ; GLYCOSIDES ; DIFFUSA
英文摘要Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction; An efficient preparative method was successfully developed for isolation and purification of unstable components from medicinal plant extracts, using a combined method of preparative high performance liquid chromatography(HPLC) and solid-phase extraction(SPE). The aim of this study was to obtain an effective method with high preparative efficiency and importantly to avoid the transformation of unstable compounds. The preparative HPLC system was based on an LC/MS controlled four-channel autopurification system. The SPE method was performed with a C(18) packing material to trap the target compounds and to remove the acidic additive derived from the mobile phase. Using this method, the unstable iridoid glucosides(IGs) as model compounds were successfully isolated and purified from the extract of Hedyotis diffusa Willd. Six IGs(including one new minor IG) and one nucleotide compound were simultaneously obtained, each with a purity of > 91% as determined by HPLC. The structures of the isolated compounds were identified by UPLC/Q-TOF MS, UV, 1D and/or 2D NMR. It was demonstrated that the combination of preparative HPLC with SPE is a versatile tool for preparative purification of unstable compounds from complex natural products.
语种英语
WOS记录号WOS:000291505100011
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115343
专题中国科学院大连化学物理研究所
通讯作者Zhang XL(张秀莉); Liang XM(梁鑫淼)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Hebei Univ Sci & Technol, Hebei Res Ctr Anal & Testing, Shijiazhuang 050018, Peoples R China
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GB/T 7714
Li Cun-man,Xiao Yuan-sheng,Xue Xing-ya,et al. Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2011,27(3):392-396.
APA Li Cun-man.,Xiao Yuan-sheng.,Xue Xing-ya.,Feng Jia-tao.,Zhang Xiu-li.,...&梁鑫淼.(2011).Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,27(3),392-396.
MLA Li Cun-man,et al."Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 27.3(2011):392-396.
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