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学科主题: 物理化学
题名: Reversed-phase depletion coupled with hydrophilic affinity enrichment for the selective isolation of N-linked glycopeptides by using Click OEG-CD matrix
作者: Zhao, Yanyan1, 2;  Yu, Long1;  Guo, Zhimou1;  Li, Xiuling1;  Liang, Xinmiao1
通讯作者: 李秀玲 ;  梁鑫淼
关键词: Click OEG-CD ;  Glycopeptide enrichment ;  Glycoproteomics ;  Hydrophilic interaction liquid chromatography
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2011-04-01
DOI: 10.1007/s00216-011-4652-9
卷: 399, 期:10, 页:3359-3365
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: Reversed-phase depletion coupled with hydrophilic affinity enrichment for the selective isolation of N-linked glycopeptides by using Click OEG-CD matrix
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Selective enrichment of glycopeptides is of great importance for protein glycosylation analysis using mass spectrometry since the signals of glycopeptides could be severely suppressed by the coexisting non-glycosylated peptides in the protein digest. In the present work, a strategy for N-linked glycopeptide enrichment through reversed-phase depletion coupled with hydrophilic affinity enrichment by applying the customized matrix named Click OEG-CD is developed. Compared with single hydrophilic interaction liquid chromatography (HILIC) mode, the strategy exhibited remarkably higher selectivity for N-linked glycopeptides. As many as 22, 18, and eight glycopeptides were detected in the glycopeptide fraction enriched with the strategy from the digests of human immunoglobulin G, horseradish peroxidase and bovine ribonuclease B, respectively. In addition, the strategy also showed high glycosylation microheterogeneity coverage for the enrichment of human alpha(1)-acid glycoprotein glycopeptides. More than 170 glycopeptides covering all the glycosylation sites were detected in the enriched fraction. The revered-phase liquid chromatography depletion coupled with HILIC enrichment strategy by using Click OEG-CD matrix is expected to show more potential in further applications in glycosylation analysis.
关键词[WOS]: MASS-SPECTROMETRY ;  LIQUID-CHROMATOGRAPHY ;  GLYCOSYLATION HETEROGENEITY ;  BORONIC ACID ;  MS ;  GLYCOPROTEINS ;  PROTEINS ;  COVERAGE ;  BINDING ;  SITES
语种: 英语
WOS记录号: WOS:000288398700005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115345
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China

Recommended Citation:
Zhao, Yanyan,Yu, Long,Guo, Zhimou,et al. Reversed-phase depletion coupled with hydrophilic affinity enrichment for the selective isolation of N-linked glycopeptides by using Click OEG-CD matrix[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(10):3359-3365.
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