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学科主题: 物理化学
题名: Purification of compounds from Lignum Dalbergia Odorifera using two-dimensional preparative chromatography with Click oligo (ethylene glycol) and C18 column
作者: Feng, Jiatao1;  Xiao, Yuansheng1;  Guo, Zhimou1;  Yu, Danhua2;  Jin, Yu2;  Liang, Xinmiao1, 2
通讯作者: 梁鑫淼
关键词: Click oligo (ethylene glycol) column ;  C18 column ;  Lignum Dalbergia Odorifera ;  Traditional Chinese medicine ;  Two-dimensional preparation
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2011-02-01
DOI: 10.1002/jssc.201000609
卷: 34, 期:3, 页:299-307
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Purification of compounds from Lignum Dalbergia Odorifera using two-dimensional preparative chromatography with Click oligo (ethylene glycol) and C18 column
研究领域[WOS]: Chemistry
英文摘要: Purification of compounds from traditional Chinese medicines (TCMs) is an important task for understanding the chemical composition of TCMs. However, it is difficult to obtain compounds with high enough purity for identification by NMR due to the complexity of TCMs in chemical composition. In this study, a two-dimensional purification method based on a Click oligo (ethylene glycol) column and a C18 column was developed to realize an orthogonal separation in preparative level for purifying compounds efficiently. The first dimensional preparation was performed on a Click oligo (ethylene glycol) column to simplify the sample into the fractions with good separation repeatability. On the first dimension, 7.2 g sample was separated into 11 fractions with a recovery of 86% within 6 h. A C18 column was taken as the second dimension to realize the high-performance separation and rapid preparation from the fractions collected from the first dimension. Eight compounds in fraction 6 and 2 compounds in fraction 8 were isolated and identified after optimizing the separation and collection parameters. This method is a high-efficient and orthogonal preparation method to improve the separation of a complex sample and increase the purity of the compounds, which benefits from the application of novel materials in the preparation and purification.
关键词[WOS]: COUNTER-CURRENT CHROMATOGRAPHY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  PHASE ;  SEPARATION
语种: 英语
WOS记录号: WOS:000287426000009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115354
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China
2.E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China

Recommended Citation:
Feng, Jiatao,Xiao, Yuansheng,Guo, Zhimou,et al. Purification of compounds from Lignum Dalbergia Odorifera using two-dimensional preparative chromatography with Click oligo (ethylene glycol) and C18 column[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(3):299-307.
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