DICP OpenIR
学科主题物理化学
Click maltose as an alternative to reverse phase material for desalting glycopeptides
Li, Juan2; Li, Xiuling1; Guo, Zhimou1; Yu, Long1; Zou, Lijuan2; Liang, Xinmiao1; Li XL(李秀玲); LijuanZou
刊名ANALYST
2011
ISSN待补充
DOI10.1039/c1an15362e
136期:19页:4075-4082
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名2,2
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MASS-SPECTROMETRY ; PROTEIN GLYCOSYLATION ; LIQUID-CHROMATOGRAPHY ; PROTEOMIC ANALYSIS ; ENRICHMENT ; MATRIX ; CANCER ; GLYCOPROTEINS ; DISEASE
英文摘要Click maltose as an alternative to reverse phase material for desalting glycopeptides; Desalting peptides before mass spectrometry analysis is important because salts lead to adduct formation, increased chemical noise and ion suppression effect. A high concentration of salt can clog nanoelectrospray ionization (ESI) emitters. The reverse phase C18 material is commonly used to desalt peptides because of its high binding capacity. However, peptides with high hydrophilicity, such as glycopeptides, are not retained well on this material, resulting in the loss of peptide information. To improve the efficiency of glycopeptide desalting, we introduced a hydrophilic interaction chromatography (HILIC)-based material named click maltose. Four glycoproteins, horseradish peroxidase (HRP), human serum immunoglobulin G (IgG), bovine ribonuclease B (RNase B), and alpha-1 acid glycoprotein (AGP) were chosen as models and their glycopeptides were desalted with click maltose, AQ C18, Empore C18 and ZipTip C18. Click maltose as a HILIC material exhibited better performance than the other three C18 materials for both number of targeted glycopeptides and their corresponding intensities. In addition, accurate glycopeptide profiling was achieved with click maltose desalting regardless of peptide lengths and glycan types.
语种英语
WOS记录号WOS:000295333100033
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115355
专题中国科学院大连化学物理研究所
通讯作者Li XL(李秀玲); LijuanZou
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116044, Peoples R China
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GB/T 7714
Li, Juan,Li, Xiuling,Guo, Zhimou,et al. Click maltose as an alternative to reverse phase material for desalting glycopeptides[J]. ANALYST,2011,136(19):4075-4082.
APA Li, Juan.,Li, Xiuling.,Guo, Zhimou.,Yu, Long.,Zou, Lijuan.,...&LijuanZou.(2011).Click maltose as an alternative to reverse phase material for desalting glycopeptides.ANALYST,136(19),4075-4082.
MLA Li, Juan,et al."Click maltose as an alternative to reverse phase material for desalting glycopeptides".ANALYST 136.19(2011):4075-4082.
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