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学科主题: 物理化学
题名: Click maltose as an alternative to reverse phase material for desalting glycopeptides
作者: Li, Juan2;  Li, Xiuling1;  Guo, Zhimou1;  Yu, Long1;  Zou, Lijuan2;  Liang, Xinmiao1
通讯作者: 李秀玲 ;  LijuanZou
刊名: ANALYST
发表日期: 2011
DOI: 10.1039/c1an15362e
卷: 136, 期:19, 页:4075-4082
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 2,2
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Click maltose as an alternative to reverse phase material for desalting glycopeptides
研究领域[WOS]: Chemistry
英文摘要: Desalting peptides before mass spectrometry analysis is important because salts lead to adduct formation, increased chemical noise and ion suppression effect. A high concentration of salt can clog nanoelectrospray ionization (ESI) emitters. The reverse phase C18 material is commonly used to desalt peptides because of its high binding capacity. However, peptides with high hydrophilicity, such as glycopeptides, are not retained well on this material, resulting in the loss of peptide information. To improve the efficiency of glycopeptide desalting, we introduced a hydrophilic interaction chromatography (HILIC)-based material named click maltose. Four glycoproteins, horseradish peroxidase (HRP), human serum immunoglobulin G (IgG), bovine ribonuclease B (RNase B), and alpha-1 acid glycoprotein (AGP) were chosen as models and their glycopeptides were desalted with click maltose, AQ C18, Empore C18 and ZipTip C18. Click maltose as a HILIC material exhibited better performance than the other three C18 materials for both number of targeted glycopeptides and their corresponding intensities. In addition, accurate glycopeptide profiling was achieved with click maltose desalting regardless of peptide lengths and glycan types.
关键词[WOS]: MASS-SPECTROMETRY ;  PROTEIN GLYCOSYLATION ;  LIQUID-CHROMATOGRAPHY ;  PROTEOMIC ANALYSIS ;  ENRICHMENT ;  MATRIX ;  CANCER ;  GLYCOPROTEINS ;  DISEASE
语种: 英语
WOS记录号: WOS:000295333100033
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115355
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116044, Peoples R China

Recommended Citation:
Li, Juan,Li, Xiuling,Guo, Zhimou,et al. Click maltose as an alternative to reverse phase material for desalting glycopeptides[J]. ANALYST,2011,136(19):4075-4082.
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