DICP OpenIR
Subject Area物理化学
Click maltose as an alternative to reverse phase material for desalting glycopeptides
Li, Juan2; Li, Xiuling1; Guo, Zhimou1; Yu, Long1; Zou, Lijuan2; Liang, Xinmiao1; Li XL(李秀玲); LijuanZou
Source PublicationANALYST
2011
ISSN待补充
DOI10.1039/c1an15362e
Volume136Issue:19Pages:4075-4082
Indexed BySCI
SubtypeArticle
Department18
Funding Project1803
Contribution Rank2,2
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMASS-SPECTROMETRY ; PROTEIN GLYCOSYLATION ; LIQUID-CHROMATOGRAPHY ; PROTEOMIC ANALYSIS ; ENRICHMENT ; MATRIX ; CANCER ; GLYCOPROTEINS ; DISEASE
AbstractClick maltose as an alternative to reverse phase material for desalting glycopeptides; Desalting peptides before mass spectrometry analysis is important because salts lead to adduct formation, increased chemical noise and ion suppression effect. A high concentration of salt can clog nanoelectrospray ionization (ESI) emitters. The reverse phase C18 material is commonly used to desalt peptides because of its high binding capacity. However, peptides with high hydrophilicity, such as glycopeptides, are not retained well on this material, resulting in the loss of peptide information. To improve the efficiency of glycopeptide desalting, we introduced a hydrophilic interaction chromatography (HILIC)-based material named click maltose. Four glycoproteins, horseradish peroxidase (HRP), human serum immunoglobulin G (IgG), bovine ribonuclease B (RNase B), and alpha-1 acid glycoprotein (AGP) were chosen as models and their glycopeptides were desalted with click maltose, AQ C18, Empore C18 and ZipTip C18. Click maltose as a HILIC material exhibited better performance than the other three C18 materials for both number of targeted glycopeptides and their corresponding intensities. In addition, accurate glycopeptide profiling was achieved with click maltose desalting regardless of peptide lengths and glycan types.
Language英语
WOS IDWOS:000295333100033
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115355
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi XL(李秀玲); LijuanZou
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116044, Peoples R China
Recommended Citation
GB/T 7714
Li, Juan,Li, Xiuling,Guo, Zhimou,et al. Click maltose as an alternative to reverse phase material for desalting glycopeptides[J]. ANALYST,2011,136(19):4075-4082.
APA Li, Juan.,Li, Xiuling.,Guo, Zhimou.,Yu, Long.,Zou, Lijuan.,...&LijuanZou.(2011).Click maltose as an alternative to reverse phase material for desalting glycopeptides.ANALYST,136(19),4075-4082.
MLA Li, Juan,et al."Click maltose as an alternative to reverse phase material for desalting glycopeptides".ANALYST 136.19(2011):4075-4082.
Files in This Item:
File Name/Size DocType Version Access License
2011tPthvoN4D6.pdf(595KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Juan]'s Articles
[Li, Xiuling]'s Articles
[Guo, Zhimou]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Juan]'s Articles
[Li, Xiuling]'s Articles
[Guo, Zhimou]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Juan]'s Articles
[Li, Xiuling]'s Articles
[Guo, Zhimou]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.