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学科主题: 物理化学
题名: Zirconia layer coated mesoporous silica microspheres as HILIC SPE materials for selective glycopeptide enrichment
作者: Wan, Huihui1;  Yan, Jingyu1;  Yu, Long1;  Sheng, Qianying2;  Zhang, Xiuli1;  Xue, Xingya1;  Li, Xiuling1;  Liang, Xinmiao1, 2
通讯作者: 李秀玲 ;  梁鑫淼
刊名: ANALYST
发表日期: 2011
DOI: 10.1039/c1an15554g
卷: 136, 期:21, 页:4422-4430
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Zirconia layer coated mesoporous silica microspheres as HILIC SPE materials for selective glycopeptide enrichment
研究领域[WOS]: Chemistry
英文摘要: Characterization of protein glycosylation requires highly specific methods for the enrichment of glycopeptides because of their sub-stoichiometric glycosylation-site occupancy. The hydrophilic affinity based strategy has attracted more attention, owing to its broad glycan specificity, good reproducibility, and compatibility with mass spectrometric (MS) analysis. Several polar matrices have emerged for hydrophilic interaction chromatography (HILIC) approaches, including sepharose, cellulose, ZIC-HILIC and titania. Here, we present the solid-phase extraction (SPE) utility of zirconia coated mesoporous silica (ZrO(2)/MPS) microspheres for glycopeptide isolation prior to MS analysis. The high specificity of this SPE approach was demonstrated by the enrichment of glycopeptides from the digests of model glycoproteins in HILIC mode. ZrO(2)/MPS microspheres show superior selectivity and glycosylation heterogeneity coverage for glycopeptide enrichment to conventional sepharose. Furthermore, digested mixtures of the phosphoprotein alpha-casein and IgG were also treated with ZrO(2)/MPS HILIC SPE materials, which exhibited that glycopeptides could be effectively enriched with interference from phosphorylated peptides.
关键词[WOS]: HYDROPHILIC-INTERACTION CHROMATOGRAPHY ;  LECTIN AFFINITY-CHROMATOGRAPHY ;  N-GLYCOSYLATED PROTEINS ;  MASS-SPECTROMETRY ;  LIQUID-CHROMATOGRAPHY ;  PHOSPHORYLATED PEPTIDES ;  FACILE SYNTHESIS ;  GLYCOPROTEINS ;  DISEASE ;  SBA-15
语种: 英语
WOS记录号: WOS:000295727900007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115358
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.E China Univ Sci & Technol, Minist Educ, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China

Recommended Citation:
Wan, Huihui,Yan, Jingyu,Yu, Long,et al. Zirconia layer coated mesoporous silica microspheres as HILIC SPE materials for selective glycopeptide enrichment[J]. ANALYST,2011,136(21):4422-4430.
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