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学科主题物理化学
Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography
Zhang, Bo2; Sheng, Qianying3; Li, Xiuling1; Liang, Qi; Yan, Jingyu1; Liang, Xinmiao1; Liang XM(梁鑫淼)
关键词C18 Fractionation Glycopeptide Hydrophilic Interaction Chromatography Mass Spectrometry Tio(2) Enrichment
刊名JOURNAL OF SEPARATION SCIENCE
2011-10-01
ISSN待补充
DOI10.1002/jssc.201100427
34期:19页:2745-2750
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名3,3
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PROTEIN GLYCOSYLATION ; LC-MS ; QUANTIFICATION ; IDENTIFICATION ; GLYCOPROTEOMICS ; STRATEGIES ; DISCOVERY
英文摘要Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography; Comprehensive glycoprotein characterization based on mass spectrometry (MS) is challenging because of low concentration of glycopeptides and suppression effect of abundant non-glycosylated peptides in MS. Therefore, it is vital to enrich glycopeptides before MS analysis. A new method was developed to selectively enrich glycopeptides from complex sample by coupling C18 fractionation with titanium dioxide (TiO(2)) enrichment. The new method allows to selectively enrich N-linked glycopeptides with various glycan forms and different sequence lengths. Compared with single TiO(2) method, the established method demonstrated higher glycopeptide selectivity and higher glycosylation heterogeneity coverage. Further application of this method to mixture of non-glycosylated protein and glycoprotein digests at different levels reveals the feasibility of enrichment of tryptic glycopeptides from simple proteomics samples.
语种英语
WOS记录号WOS:000296141200026
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115364
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Huazhong Univ Sci & Technol, Dept Gen Surg, Affiliated Union Hosp, Wuhan, Hubei Province, Peoples R China
3.E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China
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GB/T 7714
Zhang, Bo,Sheng, Qianying,Li, Xiuling,et al. Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(19):2745-2750.
APA Zhang, Bo.,Sheng, Qianying.,Li, Xiuling.,Liang, Qi.,Yan, Jingyu.,...&梁鑫淼.(2011).Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography.JOURNAL OF SEPARATION SCIENCE,34(19),2745-2750.
MLA Zhang, Bo,et al."Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography".JOURNAL OF SEPARATION SCIENCE 34.19(2011):2745-2750.
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