中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography
作者: Zhang, Bo2;  Sheng, Qianying3;  Li, Xiuling1;  Liang, Qi;  Yan, Jingyu1;  Liang, Xinmiao1
通讯作者: 梁鑫淼
关键词: C18 fractionation ;  Glycopeptide ;  Hydrophilic interaction chromatography ;  Mass spectrometry ;  TiO(2) enrichment
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2011-10-01
DOI: 10.1002/jssc.201100427
卷: 34, 期:19, 页:2745-2750
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 3,3
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography
研究领域[WOS]: Chemistry
英文摘要: Comprehensive glycoprotein characterization based on mass spectrometry (MS) is challenging because of low concentration of glycopeptides and suppression effect of abundant non-glycosylated peptides in MS. Therefore, it is vital to enrich glycopeptides before MS analysis. A new method was developed to selectively enrich glycopeptides from complex sample by coupling C18 fractionation with titanium dioxide (TiO(2)) enrichment. The new method allows to selectively enrich N-linked glycopeptides with various glycan forms and different sequence lengths. Compared with single TiO(2) method, the established method demonstrated higher glycopeptide selectivity and higher glycosylation heterogeneity coverage. Further application of this method to mixture of non-glycosylated protein and glycoprotein digests at different levels reveals the feasibility of enrichment of tryptic glycopeptides from simple proteomics samples.
关键词[WOS]: PROTEIN GLYCOSYLATION ;  LC-MS ;  QUANTIFICATION ;  IDENTIFICATION ;  GLYCOPROTEOMICS ;  STRATEGIES ;  DISCOVERY
语种: 英语
WOS记录号: WOS:000296141200026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115364
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2011OC4Ia4hOOv.pdf(288KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Huazhong Univ Sci & Technol, Dept Gen Surg, Affiliated Union Hosp, Wuhan, Hubei Province, Peoples R China
3.E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China

Recommended Citation:
Zhang, Bo,Sheng, Qianying,Li, Xiuling,et al. Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(19):2745-2750.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhang, Bo]'s Articles
 [Sheng, Qianying]'s Articles
 [Li, Xiuling]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhang, Bo]‘s Articles
 [Sheng, Qianying]‘s Articles
 [Li, Xiuling]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2011OC4Ia4hOOv.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace