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学科主题: 物理化学
题名: Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis
作者: Zhang, Biaoming1, 2;  Cao, Xupeng1;  Cheng, Xiaofei4;  Wu, Peichun1;  Xiao, Tonghu4;  Zhang, Wei1, 3
通讯作者: WeiZhang
关键词: Corallina officinalis ;  Heat treatment ;  Protein purification ;  Vanadium-dependent bromoperoxidase
刊名: BIOTECHNOLOGY LETTERS
发表日期: 2011-03-01
DOI: 10.1007/s10529-010-0454-y
卷: 33, 期:3, 页:545-548
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1812
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
摘要: Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: A novel, simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is reported. The key innovation is adding 0.5 mM sodium orthovanadate to the crude cell extract followed by heating at 70A degrees C for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. Combining the heat treatment with ammonium sulfate precipitation and DEAE-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. Finally, a specific activity of 310 U/mg, a 27-fold purification of the crude enzyme solution was produced.
关键词[WOS]: PILULIFERA
语种: 英语
WOS记录号: WOS:000287502500014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115393
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Flinders Univ S Australia, Flinders Ctr Marine Bioproc & Bioprod, Adelaide, SA 5042, Australia
4.Ningbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Peoples R China

Recommended Citation:
Zhang, Biaoming,Cao, Xupeng,Cheng, Xiaofei,et al. Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis[J]. BIOTECHNOLOGY LETTERS,2011,33(3):545-548.
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