DICP OpenIR
Subject Area物理化学
Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis
Zhang, Biaoming1,2; Cao, Xupeng1; Cheng, Xiaofei4; Wu, Peichun1; Xiao, Tonghu4; Zhang, Wei1,3; WeiZhang
KeywordCorallina Officinalis Heat Treatment Protein Purification Vanadium-dependent Bromoperoxidase
Source PublicationBIOTECHNOLOGY LETTERS
2011-03-01
ISSN待补充
DOI10.1007/s10529-010-0454-y
Volume33Issue:3Pages:545-548
Indexed BySCI
SubtypeArticle
Department18
Funding Project1812
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordPILULIFERA
AbstractEfficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis; A novel, simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is reported. The key innovation is adding 0.5 mM sodium orthovanadate to the crude cell extract followed by heating at 70A degrees C for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. Combining the heat treatment with ammonium sulfate precipitation and DEAE-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. Finally, a specific activity of 310 U/mg, a 27-fold purification of the crude enzyme solution was produced.
Language英语
WOS IDWOS:000287502500014
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115393
Collection中国科学院大连化学物理研究所
Corresponding AuthorWeiZhang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Flinders Univ S Australia, Flinders Ctr Marine Bioproc & Bioprod, Adelaide, SA 5042, Australia
4.Ningbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Biaoming,Cao, Xupeng,Cheng, Xiaofei,et al. Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis[J]. BIOTECHNOLOGY LETTERS,2011,33(3):545-548.
APA Zhang, Biaoming.,Cao, Xupeng.,Cheng, Xiaofei.,Wu, Peichun.,Xiao, Tonghu.,...&WeiZhang.(2011).Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis.BIOTECHNOLOGY LETTERS,33(3),545-548.
MLA Zhang, Biaoming,et al."Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis".BIOTECHNOLOGY LETTERS 33.3(2011):545-548.
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