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学科主题物理化学
Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis
Zhang, Biaoming1,2; Cao, Xupeng1; Cheng, Xiaofei4; Wu, Peichun1; Xiao, Tonghu4; Zhang, Wei1,3; WeiZhang
关键词Corallina Officinalis Heat Treatment Protein Purification Vanadium-dependent Bromoperoxidase
刊名BIOTECHNOLOGY LETTERS
2011-03-01
ISSN待补充
DOI10.1007/s10529-010-0454-y
33期:3页:545-548
收录类别SCI
文章类型Article
部门归属18
项目归属1812
产权排名1,1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]PILULIFERA
英文摘要Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis; A novel, simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is reported. The key innovation is adding 0.5 mM sodium orthovanadate to the crude cell extract followed by heating at 70A degrees C for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. Combining the heat treatment with ammonium sulfate precipitation and DEAE-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. Finally, a specific activity of 310 U/mg, a 27-fold purification of the crude enzyme solution was produced.
语种英语
WOS记录号WOS:000287502500014
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115393
专题中国科学院大连化学物理研究所
通讯作者WeiZhang
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Flinders Univ S Australia, Flinders Ctr Marine Bioproc & Bioprod, Adelaide, SA 5042, Australia
4.Ningbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Biaoming,Cao, Xupeng,Cheng, Xiaofei,et al. Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis[J]. BIOTECHNOLOGY LETTERS,2011,33(3):545-548.
APA Zhang, Biaoming.,Cao, Xupeng.,Cheng, Xiaofei.,Wu, Peichun.,Xiao, Tonghu.,...&WeiZhang.(2011).Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis.BIOTECHNOLOGY LETTERS,33(3),545-548.
MLA Zhang, Biaoming,et al."Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis".BIOTECHNOLOGY LETTERS 33.3(2011):545-548.
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