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学科主题: 物理化学
题名: Preparation and evaluation of hydrophilic C18 monolithic sorbents for enhanced polar compound retention in liquid chromatography and solid phase extraction
作者: Li, Yun1;  Xie, Xin1;  Lee, Milton L.2;  Chen, Jiping1
通讯作者: 陈吉平
关键词: Solid phase extraction ;  Capillary liquid chromatography ;  Monolithic C18 ;  Hydrophilic ;  Phenols
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2011-12-02
DOI: 10.1016/j.chroma.2011.09.070
卷: 1218, 期:48, 页:8608-8616
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 103
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: Preparation and evaluation of hydrophilic C18 monolithic sorbents for enhanced polar compound retention in liquid chromatography and solid phase extraction
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Hydrophilic C18 monolithic polymer sorbents were synthesized for use in solid phase extraction (SPE) and in capillary liquid chromatography (LC). The approach involved incorporating both hydrophobic and hydrophilic monomers into a monolithic material, by copolymerization of stearyl methacrylate (SMA), poly(ethylene glycol) methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EDMA) in the presence of selected porogens, to produce translucent mesoporous monolithic materials in bulk (SPE) or white macroporous monoliths inside fused silica capillary columns (capillary LC). A capillary column containing one of the hydrophilic C18 monoliths (i.e. poly(SMA-co-PEGMEMA-co-EDMA) with 15% (w/w) PEGMEMA) demonstrated nearly 35% reduction in retention of polycyclic aromatic compounds and greater than 40% increase in retention of phenols compared to a hydrophobic Cl 8 monolithic column. In addition, the hydrophilic monolith demonstrated significantly improved resolution of phenols. Similar monolithic materials prepared in bulk were ground and sieved to obtain 45-65 mu m particles with desired rigidity for SPE. To achieve optimum extraction performance for phenols, several parameters, including sample pH and volume, and eluent type and volume, were investigated. Under optimized experimental conditions, the method demonstrated good sensitivity (1.6 ng/mL LOD) and linearity (R(2) > 0.97 for 10-200 ng/mL). Again, incorporation of 15% (w/w) PEGMEMA in the monolith increased the extraction efficiency of phenols in water from approximately 20% to 67-92% compared to a hydrophobic C18 monolithic material. Increased wettability of the sorbent by the aqueous sample matrix and the presence of hydrogen-bonding interactions are responsible for the improved retention of polar compounds. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: SIZE-EXCLUSION CHROMATOGRAPHY ;  POROUS POLYMER MONOLITHS ;  MASS-SPECTROMETRY ;  STATIONARY-PHASE ;  NEW-GENERATION ;  COLUMNS ;  SEPARATION ;  ELECTROPHORESIS ;  PROTEINS ;  COATINGS
语种: 英语
WOS记录号: WOS:000297386500006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115467
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
2.Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA

Recommended Citation:
Li, Yun,Xie, Xin,Lee, Milton L.,et al. Preparation and evaluation of hydrophilic C18 monolithic sorbents for enhanced polar compound retention in liquid chromatography and solid phase extraction[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(48):8608-8616.
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