中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Electrochemical DNA biosensor for screening of chlorinated benzene pollutants
作者: Wu, Lidong;  Lu, Xianbo;  Jin, Jing;  Zhang, Haijun;  Chen, Jiping1
通讯作者: 陈吉平
关键词: Chlorinated benzenes ;  Electrochemical oligonucleotide biosensor ;  Screening ;  Toxicity evaluation ;  Environmental monitoring
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2011-06-15
DOI: 10.1016/j.bios.2011.03.027
卷: 26, 期:10, 页:4040-4045
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 103
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
摘要: Electrochemical DNA biosensor for screening of chlorinated benzene pollutants
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: In this work, an electrochemical DNA biosensor based on double-stranded DNA modified Au electrode (dsDNA/Au) was proposed for the rapid screening and detection of chlorinated benzenes pollutants, in which redox-active methylene blue (MB) was used to amplify the interaction between dsDNA and the target analyte. Using hexachlorobenzene (HCB) as a model analyte of chlorinated benzenes, the biosensor demonstrated a linear response with the logarithm of HCB concentrations from 100 pmol L(-1) to 100 nmol L(-1). The obtained detection limit was 30 pmol L(-1), which was remarkably superior to other biosensors. The interaction mechanism of the biosensor with HCB was proposed based on systematical characterization by cyclic voltammetry (CV), differential pulse voltammetry (Dry), UV-vis spectrometry and electrochemical quartz crystal microbalance (EQCM). Further studies revealed that the biosensor could screen chlorinated benzenes in the presence of 100 fold amount of other co-existing chemicals (ethyl acetate and sodium oxalate, etc.), and the response signal of the biosensors for different chlorinated benzenes was correlative to their respective toxicity. The proposed biosensor proved to be a promising "alarm" tool for rapid screening of chlorinated benzenes in real water samples. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
关键词[WOS]: CHRONIC MYELOGENOUS LEUKEMIA ;  HEPATITIS-B-VIRUS ;  METHYLENE-BLUE ;  HYBRIDIZATION INDICATOR ;  GOLD ;  MONOLAYERS ;  TRANSPORT ;  ELECTRODE ;  TOXICITY ;  QSAR
语种: 英语
WOS记录号: WOS:000291904000010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115470
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2011Lurt56JMIH.pdf(603KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Wu, Lidong,Lu, Xianbo,Jin, Jing,et al. Electrochemical DNA biosensor for screening of chlorinated benzene pollutants[J]. BIOSENSORS & BIOELECTRONICS,2011,26(10):4040-4045.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wu, Lidong]'s Articles
 [Lu, Xianbo]'s Articles
 [Jin, Jing]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wu, Lidong]‘s Articles
 [Lu, Xianbo]‘s Articles
 [Jin, Jing]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2011Lurt56JMIH.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace