DICP OpenIR
Subject Area物理化学
Electrochemical DNA biosensor for screening of chlorinated benzene pollutants
Wu, Lidong; Lu, Xianbo; Jin, Jing; Zhang, Haijun; Chen, Jiping1; Chen JP(陈吉平)
KeywordChlorinated Benzenes Electrochemical Oligonucleotide Biosensor Screening Toxicity Evaluation Environmental Monitoring
Source PublicationBIOSENSORS & BIOELECTRONICS
2011-06-15
ISSN待补充
DOI10.1016/j.bios.2011.03.027
Volume26Issue:10Pages:4040-4045
Indexed BySCI
SubtypeArticle
Department1
Funding Project103
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS KeywordCHRONIC MYELOGENOUS LEUKEMIA ; HEPATITIS-B-VIRUS ; METHYLENE-BLUE ; HYBRIDIZATION INDICATOR ; GOLD ; MONOLAYERS ; TRANSPORT ; ELECTRODE ; TOXICITY ; QSAR
AbstractElectrochemical DNA biosensor for screening of chlorinated benzene pollutants; In this work, an electrochemical DNA biosensor based on double-stranded DNA modified Au electrode (dsDNA/Au) was proposed for the rapid screening and detection of chlorinated benzenes pollutants, in which redox-active methylene blue (MB) was used to amplify the interaction between dsDNA and the target analyte. Using hexachlorobenzene (HCB) as a model analyte of chlorinated benzenes, the biosensor demonstrated a linear response with the logarithm of HCB concentrations from 100 pmol L(-1) to 100 nmol L(-1). The obtained detection limit was 30 pmol L(-1), which was remarkably superior to other biosensors. The interaction mechanism of the biosensor with HCB was proposed based on systematical characterization by cyclic voltammetry (CV), differential pulse voltammetry (Dry), UV-vis spectrometry and electrochemical quartz crystal microbalance (EQCM). Further studies revealed that the biosensor could screen chlorinated benzenes in the presence of 100 fold amount of other co-existing chemicals (ethyl acetate and sodium oxalate, etc.), and the response signal of the biosensors for different chlorinated benzenes was correlative to their respective toxicity. The proposed biosensor proved to be a promising "alarm" tool for rapid screening of chlorinated benzenes in real water samples. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000291904000010
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115470
Collection中国科学院大连化学物理研究所
Corresponding AuthorChen JP(陈吉平)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wu, Lidong,Lu, Xianbo,Jin, Jing,et al. Electrochemical DNA biosensor for screening of chlorinated benzene pollutants[J]. BIOSENSORS & BIOELECTRONICS,2011,26(10):4040-4045.
APA Wu, Lidong,Lu, Xianbo,Jin, Jing,Zhang, Haijun,Chen, Jiping,&陈吉平.(2011).Electrochemical DNA biosensor for screening of chlorinated benzene pollutants.BIOSENSORS & BIOELECTRONICS,26(10),4040-4045.
MLA Wu, Lidong,et al."Electrochemical DNA biosensor for screening of chlorinated benzene pollutants".BIOSENSORS & BIOELECTRONICS 26.10(2011):4040-4045.
Files in This Item:
File Name/Size DocType Version Access License
2011Lurt56JMIH.pdf(603KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wu, Lidong]'s Articles
[Lu, Xianbo]'s Articles
[Jin, Jing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wu, Lidong]'s Articles
[Lu, Xianbo]'s Articles
[Jin, Jing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wu, Lidong]'s Articles
[Lu, Xianbo]'s Articles
[Jin, Jing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.