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学科主题: 物理化学
题名: Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide
作者: Zhang, Hongyan1, 2;  Zhao, Xiaoming1;  Yang, Jinli1;  Yin, Heng1;  Wang, Wenxia1;  Lu, Hang1;  Du, Yuguang1
通讯作者: 赵小明 ;  杜昱光
关键词: COS ;  NO ;  OIPK ;  Defense-related enzyme ;  Tobacco
刊名: PLANT CELL REPORTS
发表日期: 2011-06-01
DOI: 10.1007/s00299-011-1024-z
卷: 30, 期:6, 页:1153-1162
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1805
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Plant Sciences
摘要: Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide
研究领域[WOS]: Plant Sciences
英文摘要: Chitooligosaccharide (COS) or oligochitosan has been shown to induce tobacco defense responses which are connected with nitric oxide (NO) and OIPK (oligochitosan-induced Ser/Thr protein kinase). The aim of this study was to reveal the relationship between NO production and OIPK pathway in the defense response of tobacco elicited by COS. NO generation was investigated by epidermal strip bioassay and fluorophore microscope using fluorophore diaminofluorescein diacetate (DAF-2DA). Tobacco epidermal cells treated with COS resulted in production of NO, which was first present in chloroplast, then in nucleus, finally in the whole cell; this NO production was sensitive to NO scavenger cPTIO and the mammalian NO synthase (NOS) inhibitor l-NAME, suggesting that NOS-like enzyme maybe involved in NO generation in tobacco epidermal cells. However, NOS and nitrate reductase (NR, EC 1.6.6.1) inhibitors reduced NO content in tobacco leaves by using NO Assay Kit, suggesting both NOS and NR were involved in NO production in tobacco leaves. Using a pharmacological approach and western blotting, we provide evidence that NO acts upstream of OIPK expression. NO scavenger, NOS inhibitor partly blocked the activation of OIPK and the activities of several defense-related enzymes induced by COS; treatment with NO donor sodium nitroprusside (SNP) induced the activation of OIPK and enhanced the defense systems. The results suggest that COS is able to induce NO generation, which results in up-regulation the activities of some defense-related enzymes through an OIPK-dependent or independent pathway.
关键词[WOS]: PLANT-DISEASE RESISTANCE ;  S-NITROSYLATED PROTEINS ;  ARABIDOPSIS-THALIANA ;  IN-VITRO ;  SUSPENSION-CULTURES ;  NITRATE REDUCTASE ;  SYNTHASE ACTIVITY ;  INNATE IMMUNITY ;  MOSAIC-VIRUS ;  NO
语种: 英语
WOS记录号: WOS:000290542800020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115510
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Zhang, Hongyan,Zhao, Xiaoming,Yang, Jinli,et al. Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide[J]. PLANT CELL REPORTS,2011,30(6):1153-1162.
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