DICP OpenIR
Subject Area物理化学
Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide
Zhang, Hongyan1,2; Zhao, Xiaoming1; Yang, Jinli1; Yin, Heng1; Wang, Wenxia1; Lu, Hang1; Du, Yuguang1; Zhao XM(赵小明); Du YG(杜昱光)
KeywordCos No Oipk Defense-related Enzyme Tobacco
Source PublicationPLANT CELL REPORTS
2011-06-01
ISSN0721-7714
DOI10.1007/s00299-011-1024-z
Volume30Issue:6Pages:1153-1162
Indexed BySCI
SubtypeArticle
Department18
Funding Project1805
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectPlant Sciences
WOS Research AreaPlant Sciences
WOS KeywordPLANT-DISEASE RESISTANCE ; S-NITROSYLATED PROTEINS ; ARABIDOPSIS-THALIANA ; IN-VITRO ; SUSPENSION-CULTURES ; NITRATE REDUCTASE ; SYNTHASE ACTIVITY ; INNATE IMMUNITY ; MOSAIC-VIRUS ; NO
AbstractNitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide; Chitooligosaccharide (COS) or oligochitosan has been shown to induce tobacco defense responses which are connected with nitric oxide (NO) and OIPK (oligochitosan-induced Ser/Thr protein kinase). The aim of this study was to reveal the relationship between NO production and OIPK pathway in the defense response of tobacco elicited by COS. NO generation was investigated by epidermal strip bioassay and fluorophore microscope using fluorophore diaminofluorescein diacetate (DAF-2DA). Tobacco epidermal cells treated with COS resulted in production of NO, which was first present in chloroplast, then in nucleus, finally in the whole cell; this NO production was sensitive to NO scavenger cPTIO and the mammalian NO synthase (NOS) inhibitor l-NAME, suggesting that NOS-like enzyme maybe involved in NO generation in tobacco epidermal cells. However, NOS and nitrate reductase (NR, EC 1.6.6.1) inhibitors reduced NO content in tobacco leaves by using NO Assay Kit, suggesting both NOS and NR were involved in NO production in tobacco leaves. Using a pharmacological approach and western blotting, we provide evidence that NO acts upstream of OIPK expression. NO scavenger, NOS inhibitor partly blocked the activation of OIPK and the activities of several defense-related enzymes induced by COS; treatment with NO donor sodium nitroprusside (SNP) induced the activation of OIPK and enhanced the defense systems. The results suggest that COS is able to induce NO generation, which results in up-regulation the activities of some defense-related enzymes through an OIPK-dependent or independent pathway.
Language英语
WOS IDWOS:000290542800020
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115510
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhao XM(赵小明); Du YG(杜昱光)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Hongyan,Zhao, Xiaoming,Yang, Jinli,et al. Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide[J]. PLANT CELL REPORTS,2011,30(6):1153-1162.
APA Zhang, Hongyan.,Zhao, Xiaoming.,Yang, Jinli.,Yin, Heng.,Wang, Wenxia.,...&杜昱光.(2011).Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide.PLANT CELL REPORTS,30(6),1153-1162.
MLA Zhang, Hongyan,et al."Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide".PLANT CELL REPORTS 30.6(2011):1153-1162.
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