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学科主题: 物理化学
题名: A bead-based approach for large-scale identification of in vitro kinase substrates
作者: Zhang, Manyu1;  Han, Guanghui1;  Wang, Chunli1;  Cheng, Kai1;  Li, Ruibin1;  Liu, Hongwei1;  Wei, Xiaoluan1;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 叶明亮 ;  邹汉法
关键词: Interaction ;  Phosphoproteomics ;  Phosphorylation ;  Protein kinases ;  Technology
刊名: PROTEOMICS
发表日期: 2011-12-01
DOI: 10.1002/pmic.201100339
卷: 11, 期:24, 页:4632-4637
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
摘要: A bead-based approach for large-scale identification of in vitro kinase substrates
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Deciphering the kinasesubstrate relationship is vital for the study of phosphorylation network. The use of immobilized proteins on protein chip as the library for screening of potential kinase substrates is a tried-and-tested method. However, information on phosphorylation sites is lacking and the creation of the library with proteins of whole proteome by recombinant expression is costly and difficult. In this study, a new solid-phase approach by immobilization of proteins from cell lysate onto beads as a protein library for kinase substrate screening was developed. It was found that consensus phosphorylation sites motif for kinase substrates could be accurately determined and hundreds of in vitro kinase substrates and their phosphorylation sites could be identified by using this method.
关键词[WOS]: PHOSPHOPROTEOME ANALYSIS ;  GLOBAL ANALYSIS ;  PHOSPHORYLATION ;  CK2 ;  CHROMATOGRAPHY ;  ENRICHMENT ;  PROTEOMICS ;  SITES ;  LIVER ;  CDK1
语种: 英语
WOS记录号: WOS:000297582900005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115568
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, Manyu,Han, Guanghui,Wang, Chunli,et al. A bead-based approach for large-scale identification of in vitro kinase substrates[J]. PROTEOMICS,2011,11(24):4632-4637.
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