DICP OpenIR
Subject Area物理化学
A bead-based approach for large-scale identification of in vitro kinase substrates
Zhang, Manyu; Han, Guanghui; Wang, Chunli; Cheng, Kai; Li, Ruibin; Liu, Hongwei; Wei, Xiaoluan; Ye, Mingliang; Zou, Hanfa; Ye ML(叶明亮); Zou HF(邹汉法)
KeywordInteraction Phosphoproteomics Phosphorylation Protein Kinases Technology
Source PublicationPROTEOMICS
2011-12-01
ISSN1615-9853
DOI10.1002/pmic.201100339
Volume11Issue:24Pages:4632-4637
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordPHOSPHOPROTEOME ANALYSIS ; GLOBAL ANALYSIS ; PHOSPHORYLATION ; CK2 ; CHROMATOGRAPHY ; ENRICHMENT ; PROTEOMICS ; SITES ; LIVER ; CDK1
AbstractA bead-based approach for large-scale identification of in vitro kinase substrates; Deciphering the kinasesubstrate relationship is vital for the study of phosphorylation network. The use of immobilized proteins on protein chip as the library for screening of potential kinase substrates is a tried-and-tested method. However, information on phosphorylation sites is lacking and the creation of the library with proteins of whole proteome by recombinant expression is costly and difficult. In this study, a new solid-phase approach by immobilization of proteins from cell lysate onto beads as a protein library for kinase substrate screening was developed. It was found that consensus phosphorylation sites motif for kinase substrates could be accurately determined and hundreds of in vitro kinase substrates and their phosphorylation sites could be identified by using this method.
Language英语
WOS IDWOS:000297582900005
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115568
Collection中国科学院大连化学物理研究所
Corresponding AuthorYe ML(叶明亮); Zou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Manyu,Han, Guanghui,Wang, Chunli,et al. A bead-based approach for large-scale identification of in vitro kinase substrates[J]. PROTEOMICS,2011,11(24):4632-4637.
APA Zhang, Manyu.,Han, Guanghui.,Wang, Chunli.,Cheng, Kai.,Li, Ruibin.,...&邹汉法.(2011).A bead-based approach for large-scale identification of in vitro kinase substrates.PROTEOMICS,11(24),4632-4637.
MLA Zhang, Manyu,et al."A bead-based approach for large-scale identification of in vitro kinase substrates".PROTEOMICS 11.24(2011):4632-4637.
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