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学科主题: 物理化学
题名: Molecularly imprinted macroporous monolithic materials for protein recognition
作者: Deng, Qi Liang1, 2;  Li, Yan Li1;  Zhang, Li Hua2;  Zhang, Yu Kui2
通讯作者: 邓启良 ;  张丽华
关键词: Molecularly imprinted polymers ;  Protein ;  Monolithic column ;  High performance liquid chromatography
刊名: CHINESE CHEMICAL LETTERS
发表日期: 2011-11-01
DOI: 10.1016/j.cclet.2011.05.044
卷: 22, 期:11, 页:1351-1354
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
摘要: Molecularly imprinted macroporous monolithic materials for protein recognition
研究领域[WOS]: Chemistry
英文摘要: Synthetic materials that can specifically recognize proteins will find wide application in many fields. In this report, bovine serum albumin was chosen as the template protein. Acrylamide and N,N'-methylenebisacrylamide were employed as the functional and cross-linker monomers, respectively. Molecularly imprinted macroporous monolithic materials that can preferentially bind the template protein in an aqueous environment were prepared by combination of molecular imprinting technique and freezing/thawing preparation method. The resulted imprinted macroporous monolithic columns were evaluated by utilizing as stationary phase in high performance liquid chromatography and solid-phase extraction materials. The experimental results indicated that the imprinted macroporous monolithic column exhibited good recognition for template protein, as compared with the control protein (hemoglobin), whereas the non-imprinted polymer (prepared under the same conditions except without addition template protein) had no selective properties. (C) 2011 Qi Liang Deng. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
关键词[WOS]: LIQUID-CHROMATOGRAPHY ;  STATIONARY PHASES ;  POLYMERS ;  ENANTIOMERS ;  SEPARATION
语种: 英语
WOS记录号: WOS:000296223400023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115644
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Tianjin Univ Sci & Technol, Dept Sci, Tianjin 300222, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Deng, Qi Liang,Li, Yan Li,Zhang, Li Hua,et al. Molecularly imprinted macroporous monolithic materials for protein recognition[J]. CHINESE CHEMICAL LETTERS,2011,22(11):1351-1354.
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