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学科主题: 物理化学
题名: Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species
作者: Gu, Qun1, 2;  David, Frank2;  Lynen, Frederic2;  Rumpel, Klaus3;  Dugardeyn, Jasper4;  Van Der Straeten, Dominique4;  Xu, Guowang1;  Sandra, Pat2
通讯作者: 许国旺 ;  SandraPat
关键词: Metabolomics ;  Gas chromatography-mass spectrometry ;  Arabidopsis thaliana ;  Multivariate statistical analysis ;  Principal component analysis ;  Mass profiler professional
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2011-05-27
DOI: 10.1016/j.chroma.2011.01.024
卷: 1218, 期:21, 页:3247-3254
收录类别: SCI ;  ISTP
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this paper, automated sample preparation, retention time locked gas chromatography-mass spectrometry (GC-MS) and data analysis methods for the metabolomics study were evaluated. A miniaturized and automated derivatisation method using sequential oximation and silylation was applied to a polar extract of 4 types (2 types x 2 ages) of Arabidopsis thaliana, a popular model organism often used in plant sciences and genetics. Automation of the derivatisation process offers excellent repeatability, and the time between sample preparation and analysis was short and constant, reducing artifact formation. Retention time locked (RTL) gas chromatography-mass spectrometry was used, resulting in reproducible retention times and GC-MS profiles. Two approaches were used for data analysis. XCMS followed by principal component analysis (approach 1) and AMDIS deconvolution combined with a commercially available program (Mass Profiler Professional) followed by principal component analysis (approach 2) were compared. Several features that were up- or down-regulated in the different types were detected. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: GC-MS ;  ASCORBIC-ACID ;  IDENTIFICATION ;  METABOLITES ;  SENESCENCE ;  POLYAMINES ;  PLANTS ;  ONSET ;  TOOL
语种: 英语
WOS记录号: WOS:000290883000018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115671
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Ghent, Pfizer Analyt Res Ctr, B-9000 Ghent, Belgium
3.Pfizer PharmaTherapeut R&D, Sandwich Labs, Sandwich CT13 9NJ, Kent, England
4.Univ Ghent, Dept Physiol, Lab Funct Plant Biol, B-9000 Ghent, Belgium

Recommended Citation:
Gu, Qun,David, Frank,Lynen, Frederic,et al. Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(21):3247-3254.
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