DICP OpenIR
学科主题物理化学
Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species
Gu, Qun1,2; David, Frank2; Lynen, Frederic2; Rumpel, Klaus3; Dugardeyn, Jasper4; Van Der Straeten, Dominique4; Xu, Guowang1; Sandra, Pat2; Xu GW(许国旺); SandraPat
关键词Metabolomics Gas Chromatography-mass Spectrometry Arabidopsis Thaliana Multivariate Statistical Analysis Principal Component Analysis Mass Profiler Professional
刊名JOURNAL OF CHROMATOGRAPHY A
2011-05-27
ISSN待补充
DOI10.1016/j.chroma.2011.01.024
1218期:21页:3247-3254
收录类别SCI ; ISTP
文章类型Article
部门归属18
项目归属1808
产权排名1,1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]GC-MS ; ASCORBIC-ACID ; IDENTIFICATION ; METABOLITES ; SENESCENCE ; POLYAMINES ; PLANTS ; ONSET ; TOOL
英文摘要Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species; In this paper, automated sample preparation, retention time locked gas chromatography-mass spectrometry (GC-MS) and data analysis methods for the metabolomics study were evaluated. A miniaturized and automated derivatisation method using sequential oximation and silylation was applied to a polar extract of 4 types (2 types x 2 ages) of Arabidopsis thaliana, a popular model organism often used in plant sciences and genetics. Automation of the derivatisation process offers excellent repeatability, and the time between sample preparation and analysis was short and constant, reducing artifact formation. Retention time locked (RTL) gas chromatography-mass spectrometry was used, resulting in reproducible retention times and GC-MS profiles. Two approaches were used for data analysis. XCMS followed by principal component analysis (approach 1) and AMDIS deconvolution combined with a commercially available program (Mass Profiler Professional) followed by principal component analysis (approach 2) were compared. Several features that were up- or down-regulated in the different types were detected. (C) 2011 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000290883000018
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115671
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺); SandraPat
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Ghent, Pfizer Analyt Res Ctr, B-9000 Ghent, Belgium
3.Pfizer PharmaTherapeut R&D, Sandwich Labs, Sandwich CT13 9NJ, Kent, England
4.Univ Ghent, Dept Physiol, Lab Funct Plant Biol, B-9000 Ghent, Belgium
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GB/T 7714
Gu, Qun,David, Frank,Lynen, Frederic,et al. Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(21):3247-3254.
APA Gu, Qun.,David, Frank.,Lynen, Frederic.,Rumpel, Klaus.,Dugardeyn, Jasper.,...&SandraPat.(2011).Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species.JOURNAL OF CHROMATOGRAPHY A,1218(21),3247-3254.
MLA Gu, Qun,et al."Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species".JOURNAL OF CHROMATOGRAPHY A 1218.21(2011):3247-3254.
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