DICP OpenIR
Subject Area物理化学
Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species
Gu, Qun1,2; David, Frank2; Lynen, Frederic2; Rumpel, Klaus3; Dugardeyn, Jasper4; Van Der Straeten, Dominique4; Xu, Guowang1; Sandra, Pat2; Xu GW(许国旺); SandraPat
KeywordMetabolomics Gas Chromatography-mass Spectrometry Arabidopsis Thaliana Multivariate Statistical Analysis Principal Component Analysis Mass Profiler Professional
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2011-05-27
ISSN待补充
DOI10.1016/j.chroma.2011.01.024
Volume1218Issue:21Pages:3247-3254
Indexed BySCI ; ISTP
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordGC-MS ; ASCORBIC-ACID ; IDENTIFICATION ; METABOLITES ; SENESCENCE ; POLYAMINES ; PLANTS ; ONSET ; TOOL
AbstractEvaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species; In this paper, automated sample preparation, retention time locked gas chromatography-mass spectrometry (GC-MS) and data analysis methods for the metabolomics study were evaluated. A miniaturized and automated derivatisation method using sequential oximation and silylation was applied to a polar extract of 4 types (2 types x 2 ages) of Arabidopsis thaliana, a popular model organism often used in plant sciences and genetics. Automation of the derivatisation process offers excellent repeatability, and the time between sample preparation and analysis was short and constant, reducing artifact formation. Retention time locked (RTL) gas chromatography-mass spectrometry was used, resulting in reproducible retention times and GC-MS profiles. Two approaches were used for data analysis. XCMS followed by principal component analysis (approach 1) and AMDIS deconvolution combined with a commercially available program (Mass Profiler Professional) followed by principal component analysis (approach 2) were compared. Several features that were up- or down-regulated in the different types were detected. (C) 2011 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000290883000018
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115671
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺); SandraPat
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Ghent, Pfizer Analyt Res Ctr, B-9000 Ghent, Belgium
3.Pfizer PharmaTherapeut R&D, Sandwich Labs, Sandwich CT13 9NJ, Kent, England
4.Univ Ghent, Dept Physiol, Lab Funct Plant Biol, B-9000 Ghent, Belgium
Recommended Citation
GB/T 7714
Gu, Qun,David, Frank,Lynen, Frederic,et al. Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(21):3247-3254.
APA Gu, Qun.,David, Frank.,Lynen, Frederic.,Rumpel, Klaus.,Dugardeyn, Jasper.,...&SandraPat.(2011).Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species.JOURNAL OF CHROMATOGRAPHY A,1218(21),3247-3254.
MLA Gu, Qun,et al."Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species".JOURNAL OF CHROMATOGRAPHY A 1218.21(2011):3247-3254.
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