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学科主题: 物理化学
题名: Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves
作者: Li, Yong1;  Pang, Tao2;  Li, Yanli1;  Wang, Xiaolin1;  Li, Qinghua1, 3;  Lu, Xin1;  Xu, Guowang1
通讯作者: 许国旺
关键词: GC-MS ;  Metabolic profiling ;  Tobacco
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2011-06-01
DOI: 10.1002/jssc.201100106
卷: 34, 期:12, 页:1447-1454
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
摘要: Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves
研究领域[WOS]: Chemistry
英文摘要: A gas chromatography-mass spectrometric method was developed for profiling of tobacco leaves. The differentiation among tobacco leaves planted in two different regions was investigated. Prior to analysis, the extraction solvent formulation was optimized and a combination of water, methanol and acetonitrile with a volume ratio of 3:1:1 was found to be optimal. The reproducibility of the method was satisfactory. Kendall tau-b rank correlation coefficients were equal to 1 (p<0.05) for 82% of the resolved peaks (up to 95% of the overall peak areas), indicating the good response correlation. Forty-four compounds including 9 saccharides, 9 alcohols, 9 amino acids, 16 organic acids and phosphoric acid were identified based on standard compounds. The method was successfully applied for profiling of tobacco leaves from Zimbabwe and Yunnan of China. Our result revealed that levels of saccharides and their derivatives including xylose, ribose, fructose, glucose, turanose, xylitol and glyceric acid were more abundant while sucrose, glucitol and D-gluconic acid were less abundant in tobacco leaves from Yunnan as compared to those from Zimbabwe. Amino acids such as L-alanine, L-tyrosine and L-threonine were found to be richer in Zimbabwe tobacco than in Yunnan tobacco.
关键词[WOS]: VOLATILE ORGANIC-ACIDS ;  MAINSTREAM CIGARETTE-SMOKE ;  MS-BASED METABOLOMICS ;  GC-MS ;  METABONOMIC ANALYSIS ;  HUMAN URINE ;  HPLC-MS ;  DERIVATIZATION ;  MICROEXTRACTION ;  OPTIMIZATION
语种: 英语
WOS记录号: WOS:000291986600011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115673
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Yunnan Acad Tobacco Agr Sci, Yuxi, Peoples R China
3.Kunming Cigarette Factory Hongyun Tobacco Grp Co, Kunming, Peoples R China

Recommended Citation:
Li, Yong,Pang, Tao,Li, Yanli,et al. Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(12):1447-1454.
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