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学科主题: 物理化学
题名: Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought
作者: Shu, Liebo1, 3;  Lou, Qiaojun1;  Ma, Chenfei2;  Ding, Wei1;  Zhou, Jia2;  Wu, Jinhong1;  Feng, Fangjun1;  Lu, Xin2;  Luo, Lijun1, 3;  Xu, Guowang2;  Mei, Hanwei1
通讯作者: 许国旺
关键词: cDNA microarray ;  Drought-induced stress ;  Metabolites ;  Oryza sativa L. ;  Plant proteomics
刊名: PROTEOMICS
发表日期: 2011-11-01
DOI: 10.1002/pmic.201000485
卷: 11, 期:21, 页:4122-4138
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 2,3
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
摘要: Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: We used proteomic analysis to determine the response of rice plant seedlings to drought-induced stress. The expression of 71 protein spots was significantly altered, and 60 spots were successfully identified. The greatest down-regulated protein functional category was translation. Up-regulated proteins were mainly related to protein folding and assembly. Additionally, many proteins involved in metabolism (e. g. carbohydrate metabolism) also showed differences in expression. cDNA microarray and GC-MS analysis showed 4756 differentially expressed mRNAs and 37 differentially expressed metabolites. Once these data were integrated with the proteomic analysis, we were able to elucidate the metabolic pathways affected by drought-induced stress. These results suggest that increased energy consumption from storage substances occurred during drought. In addition, increased expression of the enzymes involved in anabolic pathways corresponded with an increase in the content of six amino acids. We speculated that energy conversion from carbohydrates and/or fatty acids to amino acids was increased. Analysis of basic metabolism networks allowed us to understand how rice plants adjust to drought conditions.
关键词[WOS]: ORYZA-SATIVA L. ;  TRANSGENIC ARABIDOPSIS PLANTS ;  SUCROSE-PHOSPHATE SYNTHASE ;  WATER-STRESS ;  CARBOHYDRATE-METABOLISM ;  ABIOTIC STRESS ;  MAIZE LEAVES ;  PROTEIN NETWORK ;  OSMOTIC-STRESS ;  HEAT-SHOCK
语种: 英语
WOS记录号: WOS:000297223200002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115674
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanghai Agrobiol Gene Ctr, Shanghai 201106, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
3.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China

Recommended Citation:
Shu, Liebo,Lou, Qiaojun,Ma, Chenfei,et al. Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought[J]. PROTEOMICS,2011,11(21):4122-4138.
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