DICP OpenIR
Subject Area物理化学
Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought
Shu, Liebo1,3; Lou, Qiaojun1; Ma, Chenfei2; Ding, Wei1; Zhou, Jia2; Wu, Jinhong1; Feng, Fangjun1; Lu, Xin2; Luo, Lijun1,3; Xu, Guowang2; Mei, Hanwei1; Xu GW(许国旺)
KeywordCdna Microarray Drought-induced Stress Metabolites Oryza Sativa L. Plant Proteomics
Source PublicationPROTEOMICS
2011-11-01
ISSN待补充
DOI10.1002/pmic.201000485
Volume11Issue:21Pages:4122-4138
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank2,3
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordORYZA-SATIVA L. ; TRANSGENIC ARABIDOPSIS PLANTS ; SUCROSE-PHOSPHATE SYNTHASE ; WATER-STRESS ; CARBOHYDRATE-METABOLISM ; ABIOTIC STRESS ; MAIZE LEAVES ; PROTEIN NETWORK ; OSMOTIC-STRESS ; HEAT-SHOCK
AbstractGenetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought; We used proteomic analysis to determine the response of rice plant seedlings to drought-induced stress. The expression of 71 protein spots was significantly altered, and 60 spots were successfully identified. The greatest down-regulated protein functional category was translation. Up-regulated proteins were mainly related to protein folding and assembly. Additionally, many proteins involved in metabolism (e. g. carbohydrate metabolism) also showed differences in expression. cDNA microarray and GC-MS analysis showed 4756 differentially expressed mRNAs and 37 differentially expressed metabolites. Once these data were integrated with the proteomic analysis, we were able to elucidate the metabolic pathways affected by drought-induced stress. These results suggest that increased energy consumption from storage substances occurred during drought. In addition, increased expression of the enzymes involved in anabolic pathways corresponded with an increase in the content of six amino acids. We speculated that energy conversion from carbohydrates and/or fatty acids to amino acids was increased. Analysis of basic metabolism networks allowed us to understand how rice plants adjust to drought conditions.
Language英语
WOS IDWOS:000297223200002
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115674
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
Affiliation1.Shanghai Agrobiol Gene Ctr, Shanghai 201106, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
3.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Shu, Liebo,Lou, Qiaojun,Ma, Chenfei,et al. Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought[J]. PROTEOMICS,2011,11(21):4122-4138.
APA Shu, Liebo.,Lou, Qiaojun.,Ma, Chenfei.,Ding, Wei.,Zhou, Jia.,...&许国旺.(2011).Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought.PROTEOMICS,11(21),4122-4138.
MLA Shu, Liebo,et al."Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought".PROTEOMICS 11.21(2011):4122-4138.
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