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学科主题: 物理化学
题名: Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry
作者: Huang, Qiang1;  Yin, Peiyuan1;  Wang, Jing1;  Chen, Jing1;  Kong, Hongwei1;  Lu, Xin1;  Xu, Guowang1
通讯作者: 许国旺
关键词: Metabonomics ;  Liver ;  UPLC-Q-TOF/MS ;  Metabolic profiling ;  Type 2 diabetes
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2011-04-15
DOI: 10.1016/j.jchromb.2011.03.009
卷: 879, 期:13-14, 页:961-967
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
摘要: Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A protocol for the metabolic profiling of rat liver was developed based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) to explore metabolic state directly. Methanol/water (4:1, v:v) was selected as the optimal extraction solvent. The established method was validated with a linearity over the 10-5000 ng/mL for internal standards (IS) and got an average correlation coefficient of 0.9986. The intra-day and inter-day RSD for most endogenous compounds were below 15%. And the absolute recovery of IS was from 84.8% to 109.1%. Liver tissues from diabetic and control rats were enrolled in the subsequent study to show the usefulness of the method. A clear classification between the control and model animals was achieved, some significant metabolites were successfully filtered. These metabolites reflected the abnormal metabolism of diabetic rats. This initial application indicated that the method is suitable and reliable for liver tissue metabolic profiling. It is expected this protocol could also be extended to metabonomic studies of other tissues. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: FUNCTIONAL GENOMICS ;  UPLC-MS ;  STREPTOZOTOCIN ;  MELLITUS ;  OPTIMIZATION ;  SPECTROSCOPY ;  METABONOMICS ;  SAMPLES ;  URINE ;  MODEL
语种: 英语
WOS记录号: WOS:000290005400020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115680
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Huang, Qiang,Yin, Peiyuan,Wang, Jing,et al. Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(13-14):961-967.
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