Knowledge Commons of Dalian Institute of Chemical Physics, CAS
| Subject Area | 物理化学 |
| Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry | |
| Huang, Qiang; Yin, Peiyuan; Wang, Jing; Chen, Jing; Kong, Hongwei; Lu, Xin; Xu, Guowang; Xu GW(许国旺) | |
| Keyword | Metabonomics Liver Uplc-q-tof/ms Metabolic Profiling Type 2 Diabetes |
| Source Publication | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 2011-04-15 | |
| ISSN | 待补充 |
| DOI | 10.1016/j.jchromb.2011.03.009 |
| Volume | 879Issue:13-14Pages:961-967 |
| Indexed By | SCI |
| Subtype | Article |
| Department | 18 |
| Funding Project | 1808 |
| Contribution Rank | 1,1 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| WOS Subject | Biochemical Research Methods ; Chemistry, Analytical |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Keyword | FUNCTIONAL GENOMICS ; UPLC-MS ; STREPTOZOTOCIN ; MELLITUS ; OPTIMIZATION ; SPECTROSCOPY ; METABONOMICS ; SAMPLES ; URINE ; MODEL |
| Abstract | Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry; A protocol for the metabolic profiling of rat liver was developed based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) to explore metabolic state directly. Methanol/water (4:1, v:v) was selected as the optimal extraction solvent. The established method was validated with a linearity over the 10-5000 ng/mL for internal standards (IS) and got an average correlation coefficient of 0.9986. The intra-day and inter-day RSD for most endogenous compounds were below 15%. And the absolute recovery of IS was from 84.8% to 109.1%. Liver tissues from diabetic and control rats were enrolled in the subsequent study to show the usefulness of the method. A clear classification between the control and model animals was achieved, some significant metabolites were successfully filtered. These metabolites reflected the abnormal metabolism of diabetic rats. This initial application indicated that the method is suitable and reliable for liver tissue metabolic profiling. It is expected this protocol could also be extended to metabonomic studies of other tissues. (C) 2011 Elsevier B.V. All rights reserved. |
| Language | 英语 |
| WOS ID | WOS:000290005400020 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://cas-ir.dicp.ac.cn/handle/321008/115680 |
| Collection | 中国科学院大连化学物理研究所 |
| Corresponding Author | Xu GW(许国旺) |
| Affiliation | Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| Recommended Citation GB/T 7714 | Huang, Qiang,Yin, Peiyuan,Wang, Jing,et al. Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(13-14):961-967. |
| APA | Huang, Qiang.,Yin, Peiyuan.,Wang, Jing.,Chen, Jing.,Kong, Hongwei.,...&许国旺.(2011).Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(13-14),961-967. |
| MLA | Huang, Qiang,et al."Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.13-14(2011):961-967. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 2011cFn93BV5l7.pdf（940KB） | 开放获取 | -- | Application Full Text | |||
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