DICP OpenIR
Subject Area物理化学
Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry
Huang, Qiang; Yin, Peiyuan; Wang, Jing; Chen, Jing; Kong, Hongwei; Lu, Xin; Xu, Guowang; Xu GW(许国旺)
KeywordMetabonomics Liver Uplc-q-tof/ms Metabolic Profiling Type 2 Diabetes
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2011-04-15
ISSN待补充
DOI10.1016/j.jchromb.2011.03.009
Volume879Issue:13-14Pages:961-967
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordFUNCTIONAL GENOMICS ; UPLC-MS ; STREPTOZOTOCIN ; MELLITUS ; OPTIMIZATION ; SPECTROSCOPY ; METABONOMICS ; SAMPLES ; URINE ; MODEL
AbstractMethod for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry; A protocol for the metabolic profiling of rat liver was developed based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) to explore metabolic state directly. Methanol/water (4:1, v:v) was selected as the optimal extraction solvent. The established method was validated with a linearity over the 10-5000 ng/mL for internal standards (IS) and got an average correlation coefficient of 0.9986. The intra-day and inter-day RSD for most endogenous compounds were below 15%. And the absolute recovery of IS was from 84.8% to 109.1%. Liver tissues from diabetic and control rats were enrolled in the subsequent study to show the usefulness of the method. A clear classification between the control and model animals was achieved, some significant metabolites were successfully filtered. These metabolites reflected the abnormal metabolism of diabetic rats. This initial application indicated that the method is suitable and reliable for liver tissue metabolic profiling. It is expected this protocol could also be extended to metabonomic studies of other tissues. (C) 2011 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000290005400020
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115680
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Huang, Qiang,Yin, Peiyuan,Wang, Jing,et al. Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(13-14):961-967.
APA Huang, Qiang.,Yin, Peiyuan.,Wang, Jing.,Chen, Jing.,Kong, Hongwei.,...&许国旺.(2011).Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(13-14),961-967.
MLA Huang, Qiang,et al."Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.13-14(2011):961-967.
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