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学科主题: 物理化学
题名: Serum 27-nor-5 beta-Cholestane-3,7,12,24,25 Pentol Glucuronide Discovered by Metabolomics as Potential Diagnostic Biomarker for Epithelium Ovarian Cancer
作者: Chen, Jing2;  Zhang, Xiaoyan1;  Cao, Rui3;  Lu, Xin2;  Zhao, Sumin2;  Fekete, Agnes4;  Huang, Qiang2;  Schmitt-Kopplin, Philippe4;  Wang, Yisheng1;  Xu, Zhiliang2;  Wan, Xiaoping5;  Wu, Xiaohua6;  Zhao, Naiqing7;  Xu, Congjian1;  Xu, Guowang2
通讯作者: XuCongjian ;  许国旺
关键词: metabolomics ;  LC-MS ;  biomarker discovery ;  epithelium ovarian cancer ;  27-nor-5 beta-cholestane-3,7,12,24,25 pentol glucuronide
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2011-05-01
DOI: 10.1021/pr200173q
卷: 10, 期:5, 页:2625-2632
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
摘要: Serum 27-nor-5 beta-Cholestane-3,7,12,24,25 Pentol Glucuronide Discovered by Metabolomics as Potential Diagnostic Biomarker for Epithelium Ovarian Cancer
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: The aim of this study was to use a two steps strategy metabolomics to screen/identify and validate novel metabolic biomarker(s) for epithelial ovarian cancer (EOC). In the screening step, serum samples from 27 healthy women, 28 benign ovarian tumors, and 29 EOCs were analyzed by using LC-MS based nontargeted metabolomics. The three groups were separated with OSC filtered PLS-DA model, and six metabolites (27-nor-5 beta-cholestane-3,7,12,24,25 pentol glucuronide (CPG), phenylalanine, glycocholic acid, propionylcarnitine, Phe-Phe and Lyso PC (18:2)) were considered as potential biomarker candidates. In the validation step, the six metabolites were analyzed in targeted metabolomics by LC-selective ion monitoring mass spectrometry in another 685 serum samples with various clinical backgrounds. As a result, CPG was evaluated to be a potential biomarker and its content was elevated in EOC tissues compared with benign ovarian tumor tissues (p = 0.0005), Besides, CPG levels were found to be up-regulated in early stage EOC and in the three types of EOC histological types. Other variables such as nonovarian diseases, medicine consumotion, gynecological inflammations, and menopausal state did not interfere, in using CPG as diagnosis marker. CPG was found to be complementary to CA125. Our findings suggest that CPG can be considered a statistical relevant biomarker of EOC, ready for early stage detection.
关键词[WOS]: CHROMATOGRAPHY-MASS-SPECTROMETRY ;  BILE ALCOHOL ;  SPECTROSCOPIC DATA ;  HEPATITIS-B ;  METABONOMICS ;  IDENTIFICATION ;  METABOLITE ;  FUTURE ;  URINE ;  EXPRESSION
语种: 英语
WOS记录号: WOS:000290234800041
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115683
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Fudan Univ, Obstet & Gynecol Hosp, Shanghai Med Sch, Inst Biomed Sci,Shanghai Key Lab Female Reprod En, Shanghai 200011, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Dalian Med Univ, Dept Obstet & Gynecol Hosp, Dalian 116033, Peoples R China
4.Helmholtz Zentrum Muenchen, German Res Ctr Environm Hlth, Dept BioGeoChem & Analyt, Inst Ecol Chem, D-85764 Neuherberg, Germany
5.Shanghai Jiao Tong Univ, Sch Med, Int Peace Matern & Child Hlth Hosp, Shanghai 200030, Peoples R China
6.Fudan Univ, Dept Gynecol Oncol, Canc Hosp, Shanghai 200032, Peoples R China
7.Fudan Univ, Dept Biostat & Social Med, Sch Publ Hlth, Shanghai 200032, Peoples R China

Recommended Citation:
Chen, Jing,Zhang, Xiaoyan,Cao, Rui,et al. Serum 27-nor-5 beta-Cholestane-3,7,12,24,25 Pentol Glucuronide Discovered by Metabolomics as Potential Diagnostic Biomarker for Epithelium Ovarian Cancer[J]. JOURNAL OF PROTEOME RESEARCH,2011,10(5):2625-2632.
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