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学科主题物理化学
Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica
Jin, Qianru1,2; Jia, Guoqing1; Zhang, Yanmei1; Yang, Qihua1; Li, Can1; Li C(李灿)
刊名LANGMUIR
2011-10-04
ISSN待补充
DOI10.1021/la202794t
27期:19页:12016-12024
收录类别SCI
文章类型Article
部门归属5
项目归属503
产权排名1,1
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
研究领域[WOS]Chemistry ; Materials Science
关键词[WOS]EFFICIENT HETEROGENEOUS BIOCATALYSTS ; SOL-GEL-MATERIALS ; CANDIDA-ANTARCTICA ; TRYPTOPHAN FLUORESCENCE ; SECONDARY ALCOHOLS ; MESOCELLULAR FOAM ; CRYSTAL-STRUCTURE ; OPEN CONFORMATION ; ENZYME ; NANOPARTICLES
英文摘要Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica; Lipase from Pseudomonas cepacia (PCL) was successfully immobilized into siliceous mesocellular foams (MCFs) with various hydrophobic/hydrophilic surfaces. The catalytic performances of immobilized PCL were investigated using the transesterification reaction and hydrolytic reaction as model reactions. The specific activity of immobilized PCL greatly increased with enhanced surface hydrophobicity of MCFs, mainly because of lipase activation via hydrophobic interaction between alkyl groups in MCFs and the surface loop (so-called "lid") of PCL. Conformational changes of immobilized PCL were further investigated using time-resolved fluorescence spectroscopy with Trp as an intrinsic probe. When the immobilized PCL was suspended in phosphate buffer, short-lived tau(1) shortened and the fractional contribution of tau(1) significantly increased with the increasing level of surface hydrophobicity of MCFs. These results revealed that Tip(s) of the immobilized PCL were surrounded by a hydrophilic microenvironment because of the fact that the opened "lid" permitted the diffusion of water to the active site cleft. However, for the immobilized PCL suspended in n-hexane, long-lived tau(3) increased with the increase of surface hydrophobicity of MCFs. The reduced interaction between Tip(s) and the surrounding protein matrix was due to intercalation of n-hexane into the active site deft when the lipase was in open conformation. The above results demonstrated that PCL immobilized into MCF with hydrophobic surfaces were in an activated open conformation.
语种英语
WOS记录号WOS:000295187300038
引用统计
被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/115878
专题中国科学院大连化学物理研究所
通讯作者Li C(李灿)
作者单位1.Chinese Acad Sci, State Key Lab Catalysis, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Jin, Qianru,Jia, Guoqing,Zhang, Yanmei,et al. Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica[J]. LANGMUIR,2011,27(19):12016-12024.
APA Jin, Qianru,Jia, Guoqing,Zhang, Yanmei,Yang, Qihua,Li, Can,&李灿.(2011).Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica.LANGMUIR,27(19),12016-12024.
MLA Jin, Qianru,et al."Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica".LANGMUIR 27.19(2011):12016-12024.
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