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学科主题: 物理化学
题名: Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica
作者: Jin, Qianru1, 2;  Jia, Guoqing1;  Zhang, Yanmei1;  Yang, Qihua1;  Li, Can1
通讯作者: 李灿
刊名: LANGMUIR
发表日期: 2011-10-04
DOI: 10.1021/la202794t
卷: 27, 期:19, 页:12016-12024
收录类别: SCI
文章类型: Article
部门归属: 5
项目归属: 503
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Multidisciplinary ;  Chemistry, Physical ;  Materials Science, Multidisciplinary
摘要: Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica
研究领域[WOS]: Chemistry ;  Materials Science
英文摘要: Lipase from Pseudomonas cepacia (PCL) was successfully immobilized into siliceous mesocellular foams (MCFs) with various hydrophobic/hydrophilic surfaces. The catalytic performances of immobilized PCL were investigated using the transesterification reaction and hydrolytic reaction as model reactions. The specific activity of immobilized PCL greatly increased with enhanced surface hydrophobicity of MCFs, mainly because of lipase activation via hydrophobic interaction between alkyl groups in MCFs and the surface loop (so-called "lid") of PCL. Conformational changes of immobilized PCL were further investigated using time-resolved fluorescence spectroscopy with Trp as an intrinsic probe. When the immobilized PCL was suspended in phosphate buffer, short-lived tau(1) shortened and the fractional contribution of tau(1) significantly increased with the increasing level of surface hydrophobicity of MCFs. These results revealed that Tip(s) of the immobilized PCL were surrounded by a hydrophilic microenvironment because of the fact that the opened "lid" permitted the diffusion of water to the active site cleft. However, for the immobilized PCL suspended in n-hexane, long-lived tau(3) increased with the increase of surface hydrophobicity of MCFs. The reduced interaction between Tip(s) and the surrounding protein matrix was due to intercalation of n-hexane into the active site deft when the lipase was in open conformation. The above results demonstrated that PCL immobilized into MCF with hydrophobic surfaces were in an activated open conformation.
关键词[WOS]: EFFICIENT HETEROGENEOUS BIOCATALYSTS ;  SOL-GEL-MATERIALS ;  CANDIDA-ANTARCTICA ;  TRYPTOPHAN FLUORESCENCE ;  SECONDARY ALCOHOLS ;  MESOCELLULAR FOAM ;  CRYSTAL-STRUCTURE ;  OPEN CONFORMATION ;  ENZYME ;  NANOPARTICLES
语种: 英语
WOS记录号: WOS:000295187300038
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/115878
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Catalysis, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Jin, Qianru,Jia, Guoqing,Zhang, Yanmei,et al. Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica[J]. LANGMUIR,2011,27(19):12016-12024.
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