DICP OpenIR
Subject Area物理化学
Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica
Jin, Qianru1,2; Jia, Guoqing1; Zhang, Yanmei1; Yang, Qihua1; Li, Can1; Li C(李灿)
Source PublicationLANGMUIR
2011-10-04
ISSN待补充
DOI10.1021/la202794t
Volume27Issue:19Pages:12016-12024
Indexed BySCI
SubtypeArticle
Department5
Funding Project503
Contribution Rank1,1
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
WOS Research AreaChemistry ; Materials Science
WOS KeywordEFFICIENT HETEROGENEOUS BIOCATALYSTS ; SOL-GEL-MATERIALS ; CANDIDA-ANTARCTICA ; TRYPTOPHAN FLUORESCENCE ; SECONDARY ALCOHOLS ; MESOCELLULAR FOAM ; CRYSTAL-STRUCTURE ; OPEN CONFORMATION ; ENZYME ; NANOPARTICLES
AbstractHydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica; Lipase from Pseudomonas cepacia (PCL) was successfully immobilized into siliceous mesocellular foams (MCFs) with various hydrophobic/hydrophilic surfaces. The catalytic performances of immobilized PCL were investigated using the transesterification reaction and hydrolytic reaction as model reactions. The specific activity of immobilized PCL greatly increased with enhanced surface hydrophobicity of MCFs, mainly because of lipase activation via hydrophobic interaction between alkyl groups in MCFs and the surface loop (so-called "lid") of PCL. Conformational changes of immobilized PCL were further investigated using time-resolved fluorescence spectroscopy with Trp as an intrinsic probe. When the immobilized PCL was suspended in phosphate buffer, short-lived tau(1) shortened and the fractional contribution of tau(1) significantly increased with the increasing level of surface hydrophobicity of MCFs. These results revealed that Tip(s) of the immobilized PCL were surrounded by a hydrophilic microenvironment because of the fact that the opened "lid" permitted the diffusion of water to the active site cleft. However, for the immobilized PCL suspended in n-hexane, long-lived tau(3) increased with the increase of surface hydrophobicity of MCFs. The reduced interaction between Tip(s) and the surrounding protein matrix was due to intercalation of n-hexane into the active site deft when the lipase was in open conformation. The above results demonstrated that PCL immobilized into MCF with hydrophobic surfaces were in an activated open conformation.
Language英语
WOS IDWOS:000295187300038
Citation statistics
Cited Times:47[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/115878
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi C(李灿)
Affiliation1.Chinese Acad Sci, State Key Lab Catalysis, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Jin, Qianru,Jia, Guoqing,Zhang, Yanmei,et al. Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica[J]. LANGMUIR,2011,27(19):12016-12024.
APA Jin, Qianru,Jia, Guoqing,Zhang, Yanmei,Yang, Qihua,Li, Can,&李灿.(2011).Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica.LANGMUIR,27(19),12016-12024.
MLA Jin, Qianru,et al."Hydrophobic Surface Induced Activation of Pseudomonas cepacia Lipase Immobilized into Mesoporous Silica".LANGMUIR 27.19(2011):12016-12024.
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