An Integrated Debice with Combination of Protein Denaturation, Reduction, Desalting and on-column Digestion for High Throughput Pretreatment of Proteins

DICP OpenIR

学科主题	分析化学
	An Integrated Debice with Combination of Protein Denaturation, Reduction, Desalting and on-column Digestion for High Throughput Pretreatment of Proteins
	Yuan HM(袁辉明); Jiang H(蒋好); Dai ZP(戴忠鹏); Zhang LH(张丽华); Liang Z(梁振); Zhang YK(张玉奎)
会议文集	7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine
会议名称	7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine
会议日期	2011-4-15
	2011
会议地点	杭州
页码	102-1
出版者	待补充
出版地	待补充
合作性质	分会口头报告
部门归属	1810
主办者	CNHUPO IFP
英文摘要	For proteome research, "bottom-up" strategy is one of the most commonly used approaches, by which proteins are in turn denatured, reduced, alkylated, desaled and digested prior to multidimensional HPLC-ESI/MS/MS analysis. However, such a multistep sample pretreatment procedure mighet suffer from sample loss, contamination and long analysis time. Therefore, to solve these problems, it would be a better solution to develop a comprehensive on line protein pretreatment system including protein denaturation, redution, desalting and digestion.; For proteome research, "bottom-up" strategy is one of the most commonly used approaches, by which proteins are in turn denatured, reduced, alkylated, desaled and digested prior to multidimensional HPLC-ESI/MS/MS analysis. However, such a multistep sample pretreatment procedure mighet suffer from sample loss, contamination and long analysis time. Therefore, to solve these problems, it would be a better solution to develop a comprehensive on line protein pretreatment system including protein denaturation, redution, desalting and digestion.
语种	英语
文献类型	会议论文
条目标识符	http://cas-ir.dicp.ac.cn/handle/321008/116013
专题	中国科学院大连化学物理研究所
通讯作者	Zhang LH(张丽华)
推荐引用方式 GB/T 7714	Yuan HM,Jiang H,Dai ZP,et al. An Integrated Debice with Combination of Protein Denaturation, Reduction, Desalting and on-column Digestion for High Throughput Pretreatment of Proteins[C]. 待补充:待补充,2011:102-1.