| 学科主题: | 分析化学
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| 题名: | An Integrated Debice with Combination of Protein Denaturation, Reduction, Desalting and on-column Digestion for High Throughput Pretreatment of Proteins |
| 作者: | Yuan HM(袁辉明)
; Jiang H(蒋好)
; Dai ZP(戴忠鹏)
; Zhang LH(张丽华)
; Liang Z(梁振)
; Zhang YK(张玉奎)
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| 会议文集: | 7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine
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| 会议名称: | 7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine
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| 会议日期: | 2011-4-15
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| 出版日期: | 2011
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| 会议地点: | 杭州
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| 通讯作者: | 张丽华
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| 出版者: | 待补充
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| 出版地: | 待补充
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| 合作性质: | 分会口头报告
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| 部门归属: | 1810
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| 主办者: | CNHUPO IFP
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| 摘要: | For proteome research, "bottom-up" strategy is one of the most commonly used approaches, by which proteins are in turn denatured, reduced, alkylated, desaled and digested prior to multidimensional HPLC-ESI/MS/MS analysis. However, such a multistep sample pretreatment procedure mighet suffer from sample loss, contamination and long analysis time. Therefore, to solve these problems, it would be a better solution to develop a comprehensive on line protein pretreatment system including protein denaturation, redution, desalting and digestion. |
| 英文摘要: | For proteome research, "bottom-up" strategy is one of the most commonly used approaches, by which proteins are in turn denatured, reduced, alkylated, desaled and digested prior to multidimensional HPLC-ESI/MS/MS analysis. However, such a multistep sample pretreatment procedure mighet suffer from sample loss, contamination and long analysis time. Therefore, to solve these problems, it would be a better solution to develop a comprehensive on line protein pretreatment system including protein denaturation, redution, desalting and digestion. |
| 语种: | 英语
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| 内容类型: | 会议论文
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| URI标识: | http://cas-ir.dicp.ac.cn/handle/321008/116013
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| Appears in Collections: | 中国科学院大连化学物理研究所_会议论文
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| Recommended Citation: | |
Yuan HM,Jiang H,Dai ZP,et al. An Integrated Debice with Combination of Protein Denaturation, Reduction, Desalting and on-column Digestion for High Throughput Pretreatment of Proteins[C]. 见:7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine. 杭州. 2011-4-15.
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