DICP OpenIR
Subject Area分析化学
An Integrated Debice with Combination of Protein Denaturation, Reduction, Desalting and on-column Digestion for High Throughput Pretreatment of Proteins
Yuan HM(袁辉明); Jiang H(蒋好); Dai ZP(戴忠鹏); Zhang LH(张丽华); Liang Z(梁振); Zhang YK(张玉奎)
Source Publication7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine
Conference Name7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine
Conference Date2011-4-15
2011
Conference Place杭州
Pages102-1
Publisher待补充
Publication Place待补充
Cooperation Status分会口头报告
Department1810
Funding OrganizationCNHUPO IFP
AbstractFor proteome research, "bottom-up" strategy is one of the most commonly used approaches, by which proteins are in turn denatured, reduced, alkylated, desaled and digested prior to multidimensional HPLC-ESI/MS/MS analysis. However, such a multistep sample pretreatment procedure mighet suffer from sample loss, contamination and long analysis time. Therefore, to solve these problems, it would be a better solution to develop a comprehensive on line protein pretreatment system including protein denaturation, redution, desalting and digestion.; For proteome research, "bottom-up" strategy is one of the most commonly used approaches, by which proteins are in turn denatured, reduced, alkylated, desaled and digested prior to multidimensional HPLC-ESI/MS/MS analysis. However, such a multistep sample pretreatment procedure mighet suffer from sample loss, contamination and long analysis time. Therefore, to solve these problems, it would be a better solution to develop a comprehensive on line protein pretreatment system including protein denaturation, redution, desalting and digestion.
Language英语
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/116013
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Recommended Citation
GB/T 7714
Yuan HM,Jiang H,Dai ZP,et al. An Integrated Debice with Combination of Protein Denaturation, Reduction, Desalting and on-column Digestion for High Throughput Pretreatment of Proteins[C]. 待补充:待补充,2011:102-1.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[袁辉明]'s Articles
[蒋好]'s Articles
[戴忠鹏]'s Articles
Baidu academic
Similar articles in Baidu academic
[袁辉明]'s Articles
[蒋好]'s Articles
[戴忠鹏]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[袁辉明]'s Articles
[蒋好]'s Articles
[戴忠鹏]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.