Knowledge Commons of Dalian Institute of Chemical Physics, CAS
| Subject Area | 分析化学 |
| An Integrated Debice with Combination of Protein Denaturation, Reduction, Desalting and on-column Digestion for High Throughput Pretreatment of Proteins | |
| Yuan HM(袁辉明); Jiang H(蒋好); Dai ZP(戴忠鹏); Zhang LH(张丽华); Liang Z(梁振); Zhang YK(张玉奎) | |
| Source Publication | 7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine |
| Conference Name | 7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine |
| Conference Date | 2011-4-15 |
| 2011 | |
| Conference Place | 杭州 |
| Pages | 102-1 |
| Publisher | 待补充 |
| Publication Place | 待补充 |
| Cooperation Status | 分会口头报告 |
| Department | 1810 |
| Funding Organization | CNHUPO IFP |
| Abstract | For proteome research, "bottom-up" strategy is one of the most commonly used approaches, by which proteins are in turn denatured, reduced, alkylated, desaled and digested prior to multidimensional HPLC-ESI/MS/MS analysis. However, such a multistep sample pretreatment procedure mighet suffer from sample loss, contamination and long analysis time. Therefore, to solve these problems, it would be a better solution to develop a comprehensive on line protein pretreatment system including protein denaturation, redution, desalting and digestion.; For proteome research, "bottom-up" strategy is one of the most commonly used approaches, by which proteins are in turn denatured, reduced, alkylated, desaled and digested prior to multidimensional HPLC-ESI/MS/MS analysis. However, such a multistep sample pretreatment procedure mighet suffer from sample loss, contamination and long analysis time. Therefore, to solve these problems, it would be a better solution to develop a comprehensive on line protein pretreatment system including protein denaturation, redution, desalting and digestion. |
| Language | 英语 |
| Document Type | 会议论文 |
| Identifier | http://cas-ir.dicp.ac.cn/handle/321008/116013 |
| Collection | 中国科学院大连化学物理研究所 |
| Corresponding Author | Zhang LH(张丽华) |
| Recommended Citation GB/T 7714 | Yuan HM,Jiang H,Dai ZP,et al. An Integrated Debice with Combination of Protein Denaturation, Reduction, Desalting and on-column Digestion for High Throughput Pretreatment of Proteins[C]. 待补充:待补充,2011:102-1. |
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