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学科主题: 分析化学
题名: Preparation of poymer nanospheres entrapped monolithic precolumn and its application for proteome analysis
作者: Xia SM(夏思敏) ;  Yuan HM(袁辉明) ;  Jiang B(江波) ;  Liang Z(梁振) ;  Zhang LH(张丽华) ;  Zhang YK(张玉奎)
会议文集: 7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine
会议名称: 7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine
会议日期: 2011-4-15
出版日期: 2011
会议地点: 杭州
通讯作者: 张丽华
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1810
主办者: CNHUPO IFP
摘要: Due to high separation efficiency and high sensitivity, 2D-nano-HPLC-ESI/MS/MS have become one of the most popular techniques for proteome analysis. To improve analysis throughput, peptides eluted from SCX column were captured and desalted by trap column prior to RPLC separation. However, the large void volume between traditional single frit precolumn and analytical column have a negative effect on the performance of chromatrographic separation. To solve these problem, in our recent study, a polymer nanospheres entrapped monolithic precolumn was developed by entrapping P(DVB-MAA) polymer nanospheres with network of organic polymer through a photopolymerization process in capillary. The precolumns with 10cm length possess good permeability and are capable of withstanding up to 2500 psi inlet pressure, and the performance of the precolumn was evaluated by the analysis of the proteolytic digests of proteins extracted from Escherichia coli, and 93 proteins were identified , which was similar with those obtained by traditional particle packed precolumns(87). All these results demonstrated that such a nanosphere entrapped monolithic precolumn could be provide a flexible interface for construction of high throughput 2D-nano-HPLC-ESI/MS/MS.
英文摘要: Due to high separation efficiency and high sensitivity, 2D-nano-HPLC-ESI/MS/MS have become one of the most popular techniques for proteome analysis. To improve analysis throughput, peptides eluted from SCX column were captured and desalted by trap column prior to RPLC separation. However, the large void volume between traditional single frit precolumn and analytical column have a negative effect on the performance of chromatrographic separation. To solve these problem, in our recent study, a polymer nanospheres entrapped monolithic precolumn was developed by entrapping P(DVB-MAA) polymer nanospheres with network of organic polymer through a photopolymerization process in capillary. The precolumns with 10cm length possess good permeability and are capable of withstanding up to 2500 psi inlet pressure, and the performance of the precolumn was evaluated by the analysis of the proteolytic digests of proteins extracted from Escherichia coli, and 93 proteins were identified , which was similar with those obtained by traditional particle packed precolumns(87). All these results demonstrated that such a nanosphere entrapped monolithic precolumn could be provide a flexible interface for construction of high throughput 2D-nano-HPLC-ESI/MS/MS.
语种: 英语
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116015
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Xia SM,Yuan HM,Jiang B,et al. Preparation of poymer nanospheres entrapped monolithic precolumn and its application for proteome analysis[C]. 见:7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine. 杭州. 2011-4-15.
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