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学科主题: 分析化学
题名: Comparative Proteomics Analysis of Lymphatic Metastasis-associated Proteins of Highly and Low Lymphatic Metastatic Potentiality in Mouse Hepatocarcinoma Cell Lines Using Reductive Dimethylation-coupled HPLC-Chip ESI-QTOF
作者: Zhou Y(周愿) ;  Yuan HM(袁辉明) ;  Tao DY(陶定银) ;  Zhang LH(张丽华) ;  Zhang YK(张玉奎)
会议文集: 7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine
会议名称: 7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine
会议日期: 2011-4-15
出版日期: 2011
会议地点: 杭州
通讯作者: 张丽华
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1810
主办者: CNHUPO IFP
摘要: Malignant tumor is lethal because of the tumor metastasis1. So it is important to discover the biomarker of metastasis to increase the survival rate. Herein, Hca-F and Hca-P cell lines with high and low lymphatic metastasis potentiality respectively were a suitable model used in the experiment. In our experiment, the peptides labeled by the reductive dimethylation 3 (ReDi) were firstly separated by high pH RPLC; then each fraction were pooled at the same time interval2; finally the pooled fractions were analyzed by highly reproducible HPLC-CHIP-ESI-QTOF system. Relative changes between the two samples were quantified and analyzed by Mascot distiller. The result shows that 1134 protein groups were identified and 522 protein groups were quantified with more than two unique peptides. 45 proteins were up-regulated and 19 proteins were down-regulated in Hca-F cell line. In all, this method can be used for relative quantitative proteomics in the future.
英文摘要: Malignant tumor is lethal because of the tumor metastasis1. So it is important to discover the biomarker of metastasis to increase the survival rate. Herein, Hca-F and Hca-P cell lines with high and low lymphatic metastasis potentiality respectively were a suitable model used in the experiment. In our experiment, the peptides labeled by the reductive dimethylation 3 (ReDi) were firstly separated by high pH RPLC; then each fraction were pooled at the same time interval2; finally the pooled fractions were analyzed by highly reproducible HPLC-CHIP-ESI-QTOF system. Relative changes between the two samples were quantified and analyzed by Mascot distiller. The result shows that 1134 protein groups were identified and 522 protein groups were quantified with more than two unique peptides. 45 proteins were up-regulated and 19 proteins were down-regulated in Hca-F cell line. In all, this method can be used for relative quantitative proteomics in the future.
语种: 英语
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116016
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhou Y,Yuan HM,Tao DY,et al. Comparative Proteomics Analysis of Lymphatic Metastasis-associated Proteins of Highly and Low Lymphatic Metastatic Potentiality in Mouse Hepatocarcinoma Cell Lines Using Reductive Dimethylation-coupled HPLC-Chip ESI-QTOF[C]. 见:7th CNUPO Annual Congress and 3rd International Forum of Proteomics Proteomics and Translational Medicine. 杭州. 2011-4-15.
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