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学科主题: 分析化学
题名: Protein imprinted materials for Proteomic Study
作者: Zhang LH(张丽华) ;  Zhang YK(张玉奎)
会议文集: DICP Symposium (XXIX) onInternational Forum on Molecular Imprinting (2011)
会议名称: DICP Symposium (XXIX) onInternational Forum on Molecular Imprinting (2011)
会议日期: 2011-10-5
出版日期: 2011
会议地点: 大连
通讯作者: 张玉奎
出版者: 待补充
出版地: 待补充
合作性质: 特邀报告
部门归属: 1810
主办者: 大连化学物理研究所
摘要: With the recent development of proteome techniques, the interest in finding biomarkers from human plasma for clinical diagnosis has gained new momentum. However, the wide dynamic range of proteins in abundance, over 1010, brings great challenges to discover low abundance proteins with significant biological functions. [1] Therefore, in proteome study, it is indispensable to deplete high abundance proteins to identify low abundance ones. Antibody techniques have been proven the most effective methods. [1] However, with the consideration of stability, robustness and cost, the development of artificial antibody, molecularly imprinted materials, might be a state-of-the-art solution. Three generations of protein imprinted materials were developed in our study for proteomics application. In the first generation, macroporous protein-imprinted monolithic polyacrylamide materials was prepared by 3-D imprinting strategy and applied to extract the target proteins from complex protein mixtures by affinity chromatography[2]. In the second generation, one new approach, combining metal-coordination with surface imprinting technology, was developed to prepare protein-affinity materials, which showed higher specific recognition ability towards the target proteins[3]. The other new approach in the second generation, hierarchical imprinting, was developed and applied for high abundance protein imprinting. In the third generation, the epitope of the target protein was applied to overcome the difficulty in obtaining the pure protein as the template. Meanwhile, environmental-friendly polymer self-assembly technology, instead of polymerization, was applied to fabricate the materials.
英文摘要: With the recent development of proteome techniques, the interest in finding biomarkers from human plasma for clinical diagnosis has gained new momentum. However, the wide dynamic range of proteins in abundance, over 1010, brings great challenges to discover low abundance proteins with significant biological functions. [1] Therefore, in proteome study, it is indispensable to deplete high abundance proteins to identify low abundance ones. Antibody techniques have been proven the most effective methods. [1] However, with the consideration of stability, robustness and cost, the development of artificial antibody, molecularly imprinted materials, might be a state-of-the-art solution. Three generations of protein imprinted materials were developed in our study for proteomics application. In the first generation, macroporous protein-imprinted monolithic polyacrylamide materials was prepared by 3-D imprinting strategy and applied to extract the target proteins from complex protein mixtures by affinity chromatography[2]. In the second generation, one new approach, combining metal-coordination with surface imprinting technology, was developed to prepare protein-affinity materials, which showed higher specific recognition ability towards the target proteins[3]. The other new approach in the second generation, hierarchical imprinting, was developed and applied for high abundance protein imprinting. In the third generation, the epitope of the target protein was applied to overcome the difficulty in obtaining the pure protein as the template. Meanwhile, environmental-friendly polymer self-assembly technology, instead of polymerization, was applied to fabricate the materials.
语种: 英语
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116017
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhang LH,Zhang YK. Protein imprinted materials for Proteomic Study[C]. 见:DICP Symposium (XXIX) onInternational Forum on Molecular Imprinting (2011). 大连. 2011-10-5.
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