DICP OpenIR
学科主题分析化学
Protein imprinted materials for Proteomic Study
Zhang LH(张丽华); Zhang YK(张玉奎)
会议文集DICP Symposium (XXIX) onInternational Forum on Molecular Imprinting (2011)
会议名称DICP Symposium (XXIX) onInternational Forum on Molecular Imprinting (2011)
会议日期2011-10-5
2011
会议地点大连
页码33-0
出版者待补充
出版地待补充
合作性质特邀报告
部门归属1810
主办者大连化学物理研究所
英文摘要With the recent development of proteome techniques, the interest in finding biomarkers from human plasma for clinical diagnosis has gained new momentum. However, the wide dynamic range of proteins in abundance, over 1010, brings great challenges to discover low abundance proteins with significant biological functions. [1] Therefore, in proteome study, it is indispensable to deplete high abundance proteins to identify low abundance ones. Antibody techniques have been proven the most effective methods. [1] However, with the consideration of stability, robustness and cost, the development of artificial antibody, molecularly imprinted materials, might be a state-of-the-art solution. Three generations of protein imprinted materials were developed in our study for proteomics application. In the first generation, macroporous protein-imprinted monolithic polyacrylamide materials was prepared by 3-D imprinting strategy and applied to extract the target proteins from complex protein mixtures by affinity chromatography[2]. In the second generation, one new approach, combining metal-coordination with surface imprinting technology, was developed to prepare protein-affinity materials, which showed higher specific recognition ability towards the target proteins[3]. The other new approach in the second generation, hierarchical imprinting, was developed and applied for high abundance protein imprinting. In the third generation, the epitope of the target protein was applied to overcome the difficulty in obtaining the pure protein as the template. Meanwhile, environmental-friendly polymer self-assembly technology, instead of polymerization, was applied to fabricate the materials.; With the recent development of proteome techniques, the interest in finding biomarkers from human plasma for clinical diagnosis has gained new momentum. However, the wide dynamic range of proteins in abundance, over 1010, brings great challenges to discover low abundance proteins with significant biological functions. [1] Therefore, in proteome study, it is indispensable to deplete high abundance proteins to identify low abundance ones. Antibody techniques have been proven the most effective methods. [1] However, with the consideration of stability, robustness and cost, the development of artificial antibody, molecularly imprinted materials, might be a state-of-the-art solution. Three generations of protein imprinted materials were developed in our study for proteomics application. In the first generation, macroporous protein-imprinted monolithic polyacrylamide materials was prepared by 3-D imprinting strategy and applied to extract the target proteins from complex protein mixtures by affinity chromatography[2]. In the second generation, one new approach, combining metal-coordination with surface imprinting technology, was developed to prepare protein-affinity materials, which showed higher specific recognition ability towards the target proteins[3]. The other new approach in the second generation, hierarchical imprinting, was developed and applied for high abundance protein imprinting. In the third generation, the epitope of the target protein was applied to overcome the difficulty in obtaining the pure protein as the template. Meanwhile, environmental-friendly polymer self-assembly technology, instead of polymerization, was applied to fabricate the materials.
语种英语
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/116017
专题中国科学院大连化学物理研究所
通讯作者Zhang YK(张玉奎)
推荐引用方式
GB/T 7714
Zhang LH,Zhang YK. Protein imprinted materials for Proteomic Study[C]. 待补充:待补充,2011:33-0.
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