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学科主题: 分析化学
题名: Novel Separation and Identification Techniques for Proteome Study from Qualitative to Quantitative Analysis
作者: Zhang YK(张玉奎)
会议文集: 26th International Symposium on Microscale Bioseparations
会议名称: 26th International Symposium on Microscale Bioseparations
会议日期: 2011-5-1
出版日期: 2011
会议地点: 圣地亚哥
通讯作者: 张玉奎
出版者: 待补充
出版地: 待补充
合作性质: 分会特邀报告
部门归属: 1810
主办者: CASSS
摘要: In the past decade, proteomics has been seen rapid advance development due to the emerging of new analytical methods. To date, besides qualitative analysis, protein quantification, the driving force of proteomics, has been widely applied to reveal biological processes. At present, the hyphenation of isotopic labeling and mass spectrometry (MS) techniques is the dominant approach in quantitative proteomics. Many isotopic reagents were developed and applied in quantitative proteomics, such as ICAT, SILAC and iTRAQ, etc. If internal standards were added into the sample, absolute quantification information can be obtained, for example, AQUA, QconCAT etc. Besides these methods, label free and ICP-MS methods have also been commonly used for protein quantification. However, the present proteome quantitative techniques suffer from low sensitivity, low accuracy, and low throughput. Therefore, in our recent work, much effort has been made to solve these problems, by the development of new sample preparation materials with high recovery, separation matrices with high efficiency, integrated platforms with good reproducibility and high throughput, and labeling strategies with high accuracy, by which the accuracy for proteome quantitative analysis could be obviously improved.
英文摘要: In the past decade, proteomics has been seen rapid advance development due to the emerging of new analytical methods. To date, besides qualitative analysis, protein quantification, the driving force of proteomics, has been widely applied to reveal biological processes. At present, the hyphenation of isotopic labeling and mass spectrometry (MS) techniques is the dominant approach in quantitative proteomics. Many isotopic reagents were developed and applied in quantitative proteomics, such as ICAT, SILAC and iTRAQ, etc. If internal standards were added into the sample, absolute quantification information can be obtained, for example, AQUA, QconCAT etc. Besides these methods, label free and ICP-MS methods have also been commonly used for protein quantification. However, the present proteome quantitative techniques suffer from low sensitivity, low accuracy, and low throughput. Therefore, in our recent work, much effort has been made to solve these problems, by the development of new sample preparation materials with high recovery, separation matrices with high efficiency, integrated platforms with good reproducibility and high throughput, and labeling strategies with high accuracy, by which the accuracy for proteome quantitative analysis could be obviously improved.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116020
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhang YK. Novel Separation and Identification Techniques for Proteome Study from Qualitative to Quantitative Analysis[C]. 见:26th International Symposium on Microscale Bioseparations. 圣地亚哥. 2011-5-1.
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