中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 分析化学
题名: Adenosine triphosphate-functionalized magnetic nanoparticles with high selectivity for phosphopeptide enrichment
作者: Zhang LH(张丽华) ;  Zhang LY(张丽媛) ;  Sun LL(孙良亮) ;  Liang Z(梁振) ;  Zhang YK(张玉奎)
会议文集: 26th International Symposium on Microscale Bioseparations
会议名称: 26th International Symposium on Microscale Bioseparations
会议日期: 2011-5-1
出版日期: 2011
会议地点: 圣地亚哥
通讯作者: 张丽华
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1810
主办者: CASSS
摘要: Reversible protein phosphorylation plays pivotal roles in signal transduction, cell cycle, gene expression and enzymatic regulation. However, the low abundance of phosphopeptides and the signal suppression by nonphosphopeptides in MS render the comprehensive characterization of phosphoproteome still a challenge. Therefore, the selective enrichment of phosphopeptides prior to MS analysis is indispensable. Immobilized metal affinity chromatography (IMAC) is a popular approach for phosphopeptide enrichment. Usually, either iminodiacetic acid (IDA) or nitrilotriacetic aicd (NTA) was used as the ligand to immobilize metal ions. However, the nonspecific interaction between ligand and non-phosphopeptides might result in decreased enrichment specificity. With phosphate groups as ligands, the selectivity of IMAC was obviously improved. However, the applied reagents to introduce ligands, such as phosphorus oxychloride and 3-(trihydroxysilyl)propyl methylphosphate, contain only one phosphate group. In addition, such ligands were easy to hydrolysis in air, making the IMAC material preparation harsh and hard to follow. Therefore, exploiting new metal ion immobilization ligands, which could not only ensure improved the binding capacity and specificity for phosphopeptide enrichment, but also with a simple, fast and safe IMAC material preparation protocol is of great significance. In our recent work, adenosine triphosphate (ATP) with triphosphate group and biological affinity was used as the ligand to prepared IMAC materials. To achieve fast and facile phosphopeptide enrichment, amine functionalized magnetic nanoparticles (MNPs) were chosen as the matrix, and ATP was coupled to MNPs via glutaraldehyde, followed by Ti(IV) chelation. By such Ti(IV)-ATP-MNPs, 10 phosphopeptides could be effectively isolated from the digest mixture of α-casein and BSA with the molar ratio as 1:5000. Compared with the latest reported on Ti(IV) immobilized porous phosphate polymer beads[1], the selectivity was improved by 10 folds, indicating the potential of Ti(IV)-ATP-MNP in phosphoproteome research.
英文摘要: Reversible protein phosphorylation plays pivotal roles in signal transduction, cell cycle, gene expression and enzymatic regulation. However, the low abundance of phosphopeptides and the signal suppression by nonphosphopeptides in MS render the comprehensive characterization of phosphoproteome still a challenge. Therefore, the selective enrichment of phosphopeptides prior to MS analysis is indispensable. Immobilized metal affinity chromatography (IMAC) is a popular approach for phosphopeptide enrichment. Usually, either iminodiacetic acid (IDA) or nitrilotriacetic aicd (NTA) was used as the ligand to immobilize metal ions. However, the nonspecific interaction between ligand and non-phosphopeptides might result in decreased enrichment specificity. With phosphate groups as ligands, the selectivity of IMAC was obviously improved. However, the applied reagents to introduce ligands, such as phosphorus oxychloride and 3-(trihydroxysilyl)propyl methylphosphate, contain only one phosphate group. In addition, such ligands were easy to hydrolysis in air, making the IMAC material preparation harsh and hard to follow. Therefore, exploiting new metal ion immobilization ligands, which could not only ensure improved the binding capacity and specificity for phosphopeptide enrichment, but also with a simple, fast and safe IMAC material preparation protocol is of great significance. In our recent work, adenosine triphosphate (ATP) with triphosphate group and biological affinity was used as the ligand to prepared IMAC materials. To achieve fast and facile phosphopeptide enrichment, amine functionalized magnetic nanoparticles (MNPs) were chosen as the matrix, and ATP was coupled to MNPs via glutaraldehyde, followed by Ti(IV) chelation. By such Ti(IV)-ATP-MNPs, 10 phosphopeptides could be effectively isolated from the digest mixture of α-casein and BSA with the molar ratio as 1:5000. Compared with the latest reported on Ti(IV) immobilized porous phosphate polymer beads[1], the selectivity was improved by 10 folds, indicating the potential of Ti(IV)-ATP-MNP in phosphoproteome research.
语种: 英语
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116023
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Zhang LH,Zhang LY,Sun LL,et al. Adenosine triphosphate-functionalized magnetic nanoparticles with high selectivity for phosphopeptide enrichment[C]. 见:26th International Symposium on Microscale Bioseparations. 圣地亚哥. 2011-5-1.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [张丽华]'s Articles
 [张丽媛]'s Articles
 [孙良亮]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [张丽华]‘s Articles
 [张丽媛]‘s Articles
 [孙良亮]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace