DICP OpenIR
学科主题分析化学
Integrated Multidimensional Liquid Chromatography Mass Spectrometry for High Throughput Proteome Anaysis
Zhang LH(张丽华); Yuan HM(袁辉明); Zhou Y(周愿); Zhang YK(张玉奎); Liang Z(梁振); Liang ZC(梁作成)
会议文集PITTCON-the magnet for scientific innovation
会议名称Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy
会议日期2011-3-13
2011
会议地点亚特兰大
页码739-0
出版者待补充
出版地待补充
合作性质分会口头报告
部门归属1810
主办者匹兹堡光谱学会
英文摘要The complexity of proteome brings great challenge and opportunity to promote the development of new platforms for liquid phase based proteome analysis. In our recent study, to achieve high throughput, high accuracy and high sensitivity proteome analysis, 2D-SEC-micro RPLC for protein separation, a hollow fiber based interface to remove acetonitrile in the eluants of microRPLC and adjust the pH to 8.0, immobilized trypsin reactor for on-line protein digestion, and microRPLC-ESI-MS/MS for peptides separation and identification were integrated, which could be fully automatically controlled with a self-designed software. By such an integrated platform, within 21 hr, 2014 proteins were identified from rat brain with FDR<1%. Compared to the widely applied bottom up strategy, not only the analysis throughput could be obviously improved by integrating on-line protein digestion, but also the accuracy for protein identification could be increased by protein fractionation with 2D-HPLC.; The complexity of proteome brings great challenge and opportunity to promote the development of new platforms for liquid phase based proteome analysis. In our recent study, to achieve high throughput, high accuracy and high sensitivity proteome analysis, 2D-SEC-micro RPLC for protein separation, a hollow fiber based interface to remove acetonitrile in the eluants of microRPLC and adjust the pH to 8.0, immobilized trypsin reactor for on-line protein digestion, and microRPLC-ESI-MS/MS for peptides separation and identification were integrated, which could be fully automatically controlled with a self-designed software. By such an integrated platform, within 21 hr, 2014 proteins were identified from rat brain with FDR<1%. Compared to the widely applied bottom up strategy, not only the analysis throughput could be obviously improved by integrating on-line protein digestion, but also the accuracy for protein identification could be increased by protein fractionation with 2D-HPLC.
语种英语
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/116032
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
推荐引用方式
GB/T 7714
Zhang LH,Yuan HM,Zhou Y,et al. Integrated Multidimensional Liquid Chromatography Mass Spectrometry for High Throughput Proteome Anaysis[C]. 待补充:待补充,2011:739-0.
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