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学科主题: 分析化学
题名: Integrated Multidimensional Liquid Chromatography Mass Spectrometry for High Throughput Proteome Anaysis
作者: Zhang LH(张丽华) ;  Yuan HM(袁辉明) ;  Zhou Y(周愿) ;  Zhang YK(张玉奎) ;  Liang Z(梁振) ;  Liang ZC(梁作成)
会议文集: PITTCON-the magnet for scientific innovation
会议名称: Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy
会议日期: 2011-3-13
出版日期: 2011
会议地点: 亚特兰大
通讯作者: 张丽华
出版者: 待补充
出版地: 待补充
合作性质: 分会口头报告
部门归属: 1810
主办者: 匹兹堡光谱学会
摘要: The complexity of proteome brings great challenge and opportunity to promote the development of new platforms for liquid phase based proteome analysis. In our recent study, to achieve high throughput, high accuracy and high sensitivity proteome analysis, 2D-SEC-micro RPLC for protein separation, a hollow fiber based interface to remove acetonitrile in the eluants of microRPLC and adjust the pH to 8.0, immobilized trypsin reactor for on-line protein digestion, and microRPLC-ESI-MS/MS for peptides separation and identification were integrated, which could be fully automatically controlled with a self-designed software. By such an integrated platform, within 21 hr, 2014 proteins were identified from rat brain with FDR<1%. Compared to the widely applied bottom up strategy, not only the analysis throughput could be obviously improved by integrating on-line protein digestion, but also the accuracy for protein identification could be increased by protein fractionation with 2D-HPLC.
英文摘要: The complexity of proteome brings great challenge and opportunity to promote the development of new platforms for liquid phase based proteome analysis. In our recent study, to achieve high throughput, high accuracy and high sensitivity proteome analysis, 2D-SEC-micro RPLC for protein separation, a hollow fiber based interface to remove acetonitrile in the eluants of microRPLC and adjust the pH to 8.0, immobilized trypsin reactor for on-line protein digestion, and microRPLC-ESI-MS/MS for peptides separation and identification were integrated, which could be fully automatically controlled with a self-designed software. By such an integrated platform, within 21 hr, 2014 proteins were identified from rat brain with FDR<1%. Compared to the widely applied bottom up strategy, not only the analysis throughput could be obviously improved by integrating on-line protein digestion, but also the accuracy for protein identification could be increased by protein fractionation with 2D-HPLC.
语种: 英语
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116032
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhang LH,Yuan HM,Zhou Y,et al. Integrated Multidimensional Liquid Chromatography Mass Spectrometry for High Throughput Proteome Anaysis[C]. 见:Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy. 亚特兰大. 2011-3-13.
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