DICP OpenIR
Subject Area分析化学
Integrated Multidimensional Liquid Chromatography Mass Spectrometry for High Throughput Proteome Anaysis
Zhang LH(张丽华); Yuan HM(袁辉明); Zhou Y(周愿); Zhang YK(张玉奎); Liang Z(梁振); Liang ZC(梁作成)
Source PublicationPITTCON-the magnet for scientific innovation
Conference NamePittsburgh Conference on Analytical Chemistry and Applied Spectroscopy
Conference Date2011-3-13
2011
Conference Place亚特兰大
Pages739-0
Publisher待补充
Publication Place待补充
Cooperation Status分会口头报告
Department1810
Funding Organization匹兹堡光谱学会
AbstractThe complexity of proteome brings great challenge and opportunity to promote the development of new platforms for liquid phase based proteome analysis. In our recent study, to achieve high throughput, high accuracy and high sensitivity proteome analysis, 2D-SEC-micro RPLC for protein separation, a hollow fiber based interface to remove acetonitrile in the eluants of microRPLC and adjust the pH to 8.0, immobilized trypsin reactor for on-line protein digestion, and microRPLC-ESI-MS/MS for peptides separation and identification were integrated, which could be fully automatically controlled with a self-designed software. By such an integrated platform, within 21 hr, 2014 proteins were identified from rat brain with FDR<1%. Compared to the widely applied bottom up strategy, not only the analysis throughput could be obviously improved by integrating on-line protein digestion, but also the accuracy for protein identification could be increased by protein fractionation with 2D-HPLC.; The complexity of proteome brings great challenge and opportunity to promote the development of new platforms for liquid phase based proteome analysis. In our recent study, to achieve high throughput, high accuracy and high sensitivity proteome analysis, 2D-SEC-micro RPLC for protein separation, a hollow fiber based interface to remove acetonitrile in the eluants of microRPLC and adjust the pH to 8.0, immobilized trypsin reactor for on-line protein digestion, and microRPLC-ESI-MS/MS for peptides separation and identification were integrated, which could be fully automatically controlled with a self-designed software. By such an integrated platform, within 21 hr, 2014 proteins were identified from rat brain with FDR<1%. Compared to the widely applied bottom up strategy, not only the analysis throughput could be obviously improved by integrating on-line protein digestion, but also the accuracy for protein identification could be increased by protein fractionation with 2D-HPLC.
Language英语
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/116032
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Recommended Citation
GB/T 7714
Zhang LH,Yuan HM,Zhou Y,et al. Integrated Multidimensional Liquid Chromatography Mass Spectrometry for High Throughput Proteome Anaysis[C]. 待补充:待补充,2011:739-0.
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