中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 会议论文
学科主题: 微生物学
题名: Deletion of the DGAAT gene with a bleomycin resistance cassette in the oleaginous yeast Rhodosporidium toruloides
作者: Lin XP(林心萍) ;  Zhang SF(张素芳) ;  Yang F(杨帆) ;  Zhu ZW(朱志伟) ;  Zhao ZB(赵宗保)
会议文集: Book of Abstracts
会议名称: Asian Congress on Biotechnology
会议日期: 2011-5-11
出版日期: 2011
会议地点: 上海
通讯作者: 赵宗保
出版者: 待补充
出版地: 待补充
合作性质: 分会口头报告
部门归属: 1816
主办者: 亚洲生物技术联合会
摘要: The red yeast Rhodosporidium toruloides is oleaginous with a great propensity to accumulate intracellular lipids over 70% of its dry cell weight when carbohydrates were fed as carbon sources. Therefore, this yeast has been intensively studied recently in order to produce microbial lipid for a sustainable biodiesel industry. On the other hand, the biochemical machinery of lipid metabolism may be explored for production of value-added metabolites. For example, diacylglycerols, the precursor to triacylglycerols, are attractive because of their special biological activities. In this study, we isolated the genomic DNA sequence of the acyl-coenzyme A:diacylglycerol acyltransferase gene (DGAAT). We generated a knockout construction with bleomycin resistance cassette replacing the 1858 bp open reading frame of the DGAAT gene. The haploid strain R. toruloides NP11 was electroporated with the cassette, and transformant was observed on YEPD medium containing 50 μg/ml Zeocine. It should be noted that the wild-type strain were killed by 5 μg/ml Zeocine. Further PCR analysis and genome walking showed that the bleomycin cassette was integrated into the chromosome of the yeast. These results demonstrated that cassette was functional in R. toruloides under the control of the DGAAT promoter and the terminator. We will discuss more details and progress of this project during the conference.
英文摘要: The red yeast Rhodosporidium toruloides is oleaginous with a great propensity to accumulate intracellular lipids over 70% of its dry cell weight when carbohydrates were fed as carbon sources. Therefore, this yeast has been intensively studied recently in order to produce microbial lipid for a sustainable biodiesel industry. On the other hand, the biochemical machinery of lipid metabolism may be explored for production of value-added metabolites. For example, diacylglycerols, the precursor to triacylglycerols, are attractive because of their special biological activities. In this study, we isolated the genomic DNA sequence of the acyl-coenzyme A:diacylglycerol acyltransferase gene (DGAAT). We generated a knockout construction with bleomycin resistance cassette replacing the 1858 bp open reading frame of the DGAAT gene. The haploid strain R. toruloides NP11 was electroporated with the cassette, and transformant was observed on YEPD medium containing 50 μg/ml Zeocine. It should be noted that the wild-type strain were killed by 5 μg/ml Zeocine. Further PCR analysis and genome walking showed that the bleomycin cassette was integrated into the chromosome of the yeast. These results demonstrated that cassette was functional in R. toruloides under the control of the DGAAT promoter and the terminator. We will discuss more details and progress of this project during the conference.
语种: 英语
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116039
Appears in Collections:中国科学院大连化学物理研究所_会议论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Lin XP,Zhang SF,Yang F,et al. Deletion of the DGAAT gene with a bleomycin resistance cassette in the oleaginous yeast Rhodosporidium toruloides[C]. 见:Asian Congress on Biotechnology. 上海. 2011-5-11.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [林心萍]'s Articles
 [张素芳]'s Articles
 [杨帆]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [林心萍]‘s Articles
 [张素芳]‘s Articles
 [杨帆]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace