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学科主题: 有机化学工程
题名: Enzymatic Hydrolysates of Corn Stover Pretreated by the Ionic Liquids/N-Methylpyrrolidone Solution for Microbial Lipid Production
作者: Xie HB(谢海波) ;  Shen HW(沈宏伟) ;  Gong ZW(龚志伟) ;  Wang Q(王倩) ;  Zhao ZB(赵宗保) ;  Bai FW(白凤武)
会议文集: The 6th Sino-US Conference of Chemical Engineering
会议名称: The 6th Sino-US Joint Conference of Chemical Engineering
会议日期: 2011-11-7
出版日期: 2011
会议地点: 北京
通讯作者: 谢海波 ;  赵宗保
出版者: 待补充
出版地: 待补充
合作性质: 分会口头报告
部门归属: 1816
主办者: 中国石化,经济与发展研究所
摘要: Efficient transformation of lignocellulosic materials into sugars has been intensively pursued for the production of biofuels and chemicals by microbial conversion.[1] In this study, we show that corn stover can be effectively dissolved in the solution of ionic liquids in N-methylpyrrolidone (NMP), and that the enzymatic hydrolysates of the regenerated materials are suitable for microbial lipid production by the oleaginous yeast Rhodosporidium Toruloides Y4 (R. toruloides Y4). Up to 10 wt% corn stover was dissolved in EmimAc/NMP (XILs=0.2, mole fraction of ionic liquids in the mixed solution) at 140 °C after 60 min. Physiochemical analysis showed major differences in terms of cellulose crystallinity, compositional distribution and surface morphology between raw corn stover samples and the regenerated materials, indicating that dissolution in the EmimAc/NMP system followed by regeneration with anti-solvents could be used as an effective pretreatment technology. Enzymatic saccharification of the regenerated corn stover afforded an 82.9% total reducing sugars yield and a 60.8% glucose yield within 24 h. The hydrolysates were used, without detoxification, as the carbon sources for the cultivation of R. toruloides Y4 for lipid production. Glucose and xylose in the hydrolysates were both consumed in our process. The overall lipid yield was estimated as 0.071 g lipid per g raw corn stover, but it is expected to be greatly improved in the future. Our study provides an integrated process to convert lignocellulosic biomass into lipid and should be an appealing route for further optimization and development.
英文摘要: Efficient transformation of lignocellulosic materials into sugars has been intensively pursued for the production of biofuels and chemicals by microbial conversion.[1] In this study, we show that corn stover can be effectively dissolved in the solution of ionic liquids in N-methylpyrrolidone (NMP), and that the enzymatic hydrolysates of the regenerated materials are suitable for microbial lipid production by the oleaginous yeast Rhodosporidium Toruloides Y4 (R. toruloides Y4). Up to 10 wt% corn stover was dissolved in EmimAc/NMP (XILs=0.2, mole fraction of ionic liquids in the mixed solution) at 140 °C after 60 min. Physiochemical analysis showed major differences in terms of cellulose crystallinity, compositional distribution and surface morphology between raw corn stover samples and the regenerated materials, indicating that dissolution in the EmimAc/NMP system followed by regeneration with anti-solvents could be used as an effective pretreatment technology. Enzymatic saccharification of the regenerated corn stover afforded an 82.9% total reducing sugars yield and a 60.8% glucose yield within 24 h. The hydrolysates were used, without detoxification, as the carbon sources for the cultivation of R. toruloides Y4 for lipid production. Glucose and xylose in the hydrolysates were both consumed in our process. The overall lipid yield was estimated as 0.071 g lipid per g raw corn stover, but it is expected to be greatly improved in the future. Our study provides an integrated process to convert lignocellulosic biomass into lipid and should be an appealing route for further optimization and development.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116045
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Xie HB,Shen HW,Gong ZW,et al. Enzymatic Hydrolysates of Corn Stover Pretreated by the Ionic Liquids/N-Methylpyrrolidone Solution for Microbial Lipid Production[C]. 见:The 6th Sino-US Joint Conference of Chemical Engineering. 北京. 2011-11-7.
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