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学科主题: 分析化学
题名: PREPARATION MAGNETIC MESOPOROUS MATERIAL USED FOR SELECTIVE ENRICHMENT OF IMPORTANT CIS-DIOL-CONTAINING BIOMOLECULES
作者: Li H(李华) ;  Shi XZ(石先哲) ;  Lu X(路鑫) ;  Xu GW(许国旺)
会议文集: Proceeding of BCEIA 2011
会议名称: 14th Beijing Conference and Exhibition on Instrumental Analysis
会议日期: 2011-10-12
出版日期: 2011
会议地点: 北京
通讯作者: 许国旺
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1808
主办者: 中国分析测试学会
摘要: Some cis-diol-containing biomolecules such as glycoproteins, modified nucleosides are closely linked with biological processes such as cellular recognition, proliferation. What’s more important, some of them are biomarkers of some illnesses such as malignant tumor, and the fluctuation of their content is closely related to the lesion progression. Unfortunately, detection of the cis-diol-containing biomolecules is affected seriously by other compounds which coexist with them in body fluid abundantly, in addition most of the cis-diol-containing biomolecules are existing in trace amount. Obviously detection is difficult so selective enrichment is necessary. In recent decades, great efforts have been paid to selective enrichment cis-diol-containing biomolecules. Typically, commercialized agarose-bead -bound m-aminophenyl boronic acid is a kind of favorite SPE stationary phase however it has to be controlled in alkaline media while capture and in acid media while release. As we know, the physiological PH is 7.4, so it is convenient if affinity can be realized in neutral PH , and introducing a neighboring amino group to form wulff-type boronic acid or an electron-withdrawing group into the molecular can achieve such a goal. Here we represent a kind of core-shell structure magnetic mesoporous material which is composed of mesoporous silica and magnetic nanoparticles. Boronic acid affinity groups were adorned into the tunnel of mesoporous with 1,6-hexanediamine together to realize capture/release in physiological PH. Mesoporous can supply size exclusion effects for cis-diol-containing biomolecules against other macromolecules and enlarge surface area at the same time. The magnetic nanoparticles could respond to external magnetic field rapidly so it will be convenient for the processes of pretreatment. TEM showed that mesoporous was regularly arranged, and N2 adsorption demonstrated that surface area was about 698 m2/g and average pore diameter was about 2.2nm. FT-IR was used to prove the boronic acid groups have been introduced successfully. The mixture of modified nucleosides was used to test the selectivity of the new material.
英文摘要: Some cis-diol-containing biomolecules such as glycoproteins, modified nucleosides are closely linked with biological processes such as cellular recognition, proliferation. What’s more important, some of them are biomarkers of some illnesses such as malignant tumor, and the fluctuation of their content is closely related to the lesion progression. Unfortunately, detection of the cis-diol-containing biomolecules is affected seriously by other compounds which coexist with them in body fluid abundantly, in addition most of the cis-diol-containing biomolecules are existing in trace amount. Obviously detection is difficult so selective enrichment is necessary. In recent decades, great efforts have been paid to selective enrichment cis-diol-containing biomolecules. Typically, commercialized agarose-bead -bound m-aminophenyl boronic acid is a kind of favorite SPE stationary phase however it has to be controlled in alkaline media while capture and in acid media while release. As we know, the physiological PH is 7.4, so it is convenient if affinity can be realized in neutral PH , and introducing a neighboring amino group to form wulff-type boronic acid or an electron-withdrawing group into the molecular can achieve such a goal. Here we represent a kind of core-shell structure magnetic mesoporous material which is composed of mesoporous silica and magnetic nanoparticles. Boronic acid affinity groups were adorned into the tunnel of mesoporous with 1,6-hexanediamine together to realize capture/release in physiological PH. Mesoporous can supply size exclusion effects for cis-diol-containing biomolecules against other macromolecules and enlarge surface area at the same time. The magnetic nanoparticles could respond to external magnetic field rapidly so it will be convenient for the processes of pretreatment. TEM showed that mesoporous was regularly arranged, and N2 adsorption demonstrated that surface area was about 698 m2/g and average pore diameter was about 2.2nm. FT-IR was used to prove the boronic acid groups have been introduced successfully. The mixture of modified nucleosides was used to test the selectivity of the new material.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116048
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Li H,Shi XZ,Lu X,et al. PREPARATION MAGNETIC MESOPOROUS MATERIAL USED FOR SELECTIVE ENRICHMENT OF IMPORTANT CIS-DIOL-CONTAINING BIOMOLECULES[C]. 见:14th Beijing Conference and Exhibition on Instrumental Analysis. 北京. 2011-10-12.
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