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学科主题分析化学
DANSYLATION DERIVATIZATION ENHANCED THE DETECTION OF AMINO ACIDS BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY
Dai WD(戴伟东); Zhao CX(赵春霞); Kong HW(孔宏伟); Lu X(路鑫); Xu GW(许国旺)
会议文集Proceeding of BCEIA 2011
会议名称14th Beijing Conference and Exhibition on Instrumental Analysis
会议日期2011-10-12
2011
会议地点北京
页码529-1
出版者待补充
出版地待补充
合作性质墙报
部门归属1808
主办者中国分析测试学会
英文摘要High polar metabolites, containing carbohydrates, amino acids and their derivatives, and nucleosides etc, exist abundantly in plasma, urine and intracellular fluid. However, due to the poor retention on reversed-phase (RP) columns and the severe ion suppression, it is difficult to quantify these metabolites accurately by liquid chromatography mass spectrometry (LC-MS) using RP C18 columns in metabonomics studies. Derivatization is a commonly used means to improve sensitivity for specific compounds when use UV or fluorescence detectors, and was applied into LC-MS analysis currently. In this study, dansyl chloride was employed to react with amino acids. 19 amino acids were selected to evaluate the performance of dansylated amino acids by LC-MS. The results (Figure 1) showed that dansylation prolonged the retention times of amino acids on C18 column (changed from 1-9.5 min to 15-22 min) and enhanced the sensitivity markedly. Such benefits were attributed to three major factors: 1) dansylation of amino acids improved ionization efficiency; 2) dansylation of amino acids increased the hydrophobicity of compounds; 3) elution with high organic phase leaded to the greater nebulization efficiency.; High polar metabolites, containing carbohydrates, amino acids and their derivatives, and nucleosides etc, exist abundantly in plasma, urine and intracellular fluid. However, due to the poor retention on reversed-phase (RP) columns and the severe ion suppression, it is difficult to quantify these metabolites accurately by liquid chromatography mass spectrometry (LC-MS) using RP C18 columns in metabonomics studies. Derivatization is a commonly used means to improve sensitivity for specific compounds when use UV or fluorescence detectors, and was applied into LC-MS analysis currently. In this study, dansyl chloride was employed to react with amino acids. 19 amino acids were selected to evaluate the performance of dansylated amino acids by LC-MS. The results (Figure 1) showed that dansylation prolonged the retention times of amino acids on C18 column (changed from 1-9.5 min to 15-22 min) and enhanced the sensitivity markedly. Such benefits were attributed to three major factors: 1) dansylation of amino acids improved ionization efficiency; 2) dansylation of amino acids increased the hydrophobicity of compounds; 3) elution with high organic phase leaded to the greater nebulization efficiency.
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/116050
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺)
推荐引用方式
GB/T 7714
Dai WD,Zhao CX,Kong HW,et al. DANSYLATION DERIVATIZATION ENHANCED THE DETECTION OF AMINO ACIDS BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY[C]. 待补充:待补充,2011:529-1.
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