DICP OpenIR
Subject Area分析化学
DANSYLATION DERIVATIZATION ENHANCED THE DETECTION OF AMINO ACIDS BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY
Dai WD(戴伟东); Zhao CX(赵春霞); Kong HW(孔宏伟); Lu X(路鑫); Xu GW(许国旺)
Source PublicationProceeding of BCEIA 2011
Conference Name14th Beijing Conference and Exhibition on Instrumental Analysis
Conference Date2011-10-12
2011
Conference Place北京
Pages529-1
Publisher待补充
Publication Place待补充
Cooperation Status墙报
Department1808
Funding Organization中国分析测试学会
AbstractHigh polar metabolites, containing carbohydrates, amino acids and their derivatives, and nucleosides etc, exist abundantly in plasma, urine and intracellular fluid. However, due to the poor retention on reversed-phase (RP) columns and the severe ion suppression, it is difficult to quantify these metabolites accurately by liquid chromatography mass spectrometry (LC-MS) using RP C18 columns in metabonomics studies. Derivatization is a commonly used means to improve sensitivity for specific compounds when use UV or fluorescence detectors, and was applied into LC-MS analysis currently. In this study, dansyl chloride was employed to react with amino acids. 19 amino acids were selected to evaluate the performance of dansylated amino acids by LC-MS. The results (Figure 1) showed that dansylation prolonged the retention times of amino acids on C18 column (changed from 1-9.5 min to 15-22 min) and enhanced the sensitivity markedly. Such benefits were attributed to three major factors: 1) dansylation of amino acids improved ionization efficiency; 2) dansylation of amino acids increased the hydrophobicity of compounds; 3) elution with high organic phase leaded to the greater nebulization efficiency.; High polar metabolites, containing carbohydrates, amino acids and their derivatives, and nucleosides etc, exist abundantly in plasma, urine and intracellular fluid. However, due to the poor retention on reversed-phase (RP) columns and the severe ion suppression, it is difficult to quantify these metabolites accurately by liquid chromatography mass spectrometry (LC-MS) using RP C18 columns in metabonomics studies. Derivatization is a commonly used means to improve sensitivity for specific compounds when use UV or fluorescence detectors, and was applied into LC-MS analysis currently. In this study, dansyl chloride was employed to react with amino acids. 19 amino acids were selected to evaluate the performance of dansylated amino acids by LC-MS. The results (Figure 1) showed that dansylation prolonged the retention times of amino acids on C18 column (changed from 1-9.5 min to 15-22 min) and enhanced the sensitivity markedly. Such benefits were attributed to three major factors: 1) dansylation of amino acids improved ionization efficiency; 2) dansylation of amino acids increased the hydrophobicity of compounds; 3) elution with high organic phase leaded to the greater nebulization efficiency.
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/116050
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
Recommended Citation
GB/T 7714
Dai WD,Zhao CX,Kong HW,et al. DANSYLATION DERIVATIZATION ENHANCED THE DETECTION OF AMINO ACIDS BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY[C]. 待补充:待补充,2011:529-1.
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