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学科主题: 分析化学
题名: DANSYLATION DERIVATIZATION ENHANCED THE DETECTION OF AMINO ACIDS BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY
作者: Dai WD(戴伟东) ;  Zhao CX(赵春霞) ;  Kong HW(孔宏伟) ;  Lu X(路鑫) ;  Xu GW(许国旺)
会议文集: Proceeding of BCEIA 2011
会议名称: 14th Beijing Conference and Exhibition on Instrumental Analysis
会议日期: 2011-10-12
出版日期: 2011
会议地点: 北京
通讯作者: 许国旺
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1808
主办者: 中国分析测试学会
摘要: High polar metabolites, containing carbohydrates, amino acids and their derivatives, and nucleosides etc, exist abundantly in plasma, urine and intracellular fluid. However, due to the poor retention on reversed-phase (RP) columns and the severe ion suppression, it is difficult to quantify these metabolites accurately by liquid chromatography mass spectrometry (LC-MS) using RP C18 columns in metabonomics studies. Derivatization is a commonly used means to improve sensitivity for specific compounds when use UV or fluorescence detectors, and was applied into LC-MS analysis currently. In this study, dansyl chloride was employed to react with amino acids. 19 amino acids were selected to evaluate the performance of dansylated amino acids by LC-MS. The results (Figure 1) showed that dansylation prolonged the retention times of amino acids on C18 column (changed from 1-9.5 min to 15-22 min) and enhanced the sensitivity markedly. Such benefits were attributed to three major factors: 1) dansylation of amino acids improved ionization efficiency; 2) dansylation of amino acids increased the hydrophobicity of compounds; 3) elution with high organic phase leaded to the greater nebulization efficiency.
英文摘要: High polar metabolites, containing carbohydrates, amino acids and their derivatives, and nucleosides etc, exist abundantly in plasma, urine and intracellular fluid. However, due to the poor retention on reversed-phase (RP) columns and the severe ion suppression, it is difficult to quantify these metabolites accurately by liquid chromatography mass spectrometry (LC-MS) using RP C18 columns in metabonomics studies. Derivatization is a commonly used means to improve sensitivity for specific compounds when use UV or fluorescence detectors, and was applied into LC-MS analysis currently. In this study, dansyl chloride was employed to react with amino acids. 19 amino acids were selected to evaluate the performance of dansylated amino acids by LC-MS. The results (Figure 1) showed that dansylation prolonged the retention times of amino acids on C18 column (changed from 1-9.5 min to 15-22 min) and enhanced the sensitivity markedly. Such benefits were attributed to three major factors: 1) dansylation of amino acids improved ionization efficiency; 2) dansylation of amino acids increased the hydrophobicity of compounds; 3) elution with high organic phase leaded to the greater nebulization efficiency.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116050
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Dai WD,Zhao CX,Kong HW,et al. DANSYLATION DERIVATIZATION ENHANCED THE DETECTION OF AMINO ACIDS BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY[C]. 见:14th Beijing Conference and Exhibition on Instrumental Analysis. 北京. 2011-10-12.
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