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学科主题: 分析化学
题名: On-Line Micro Solid-Phase Extraction Combined with Capillary Liquid Chromatography-Tandem Mass Spectrometry for High Throughput Analysis of Microcystins and Nodularin-R
作者: Dan YH(单圆鸿) ;  Shi XZ(石先哲) ;  Xu GW(许国旺)
会议文集: 36th International Symposium on High Performance Liquid Phase Separations and Related Techniques
会议名称: 36th International Symposium on High Performance Liquid Phase Separations and Related Techniques
会议日期: 2011-6-19
出版日期: 2011
会议地点: 布达佩斯
通讯作者: 许国旺
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1808
主办者: Hungarian Society for Separation Sciences
摘要: Microcystins and nodularins are cyclic peptide hepatotoxins and tumour promoters from cyanobacteria. The present study describes the development, validation and practical application of a fully automated analytical method based on on-line micro solid-phase extraction-capillary liquid chromatography-tandem mass spectrometry for the simultaneous determination of seven microcystins and nodularin-R in tap water and lake water. Aliquots of just 100 μL of water samples are sufficient for the detection and quantification of all eight toxins. Selected reaction monitoring was used to obtain the highest sensitivity. Good linear calibrations were obtained for microcystins (50–2000 ng/L) and nodularin-R (25–1000 ng/L) in spiked tap water and lake water samples. Excellent interday and intraday repeatability were achieved for eight toxins with relative standard deviation less than 15.7% in three different concentrations. Acceptable recoveries were achieved in the three concentrations with both tap water matrix and lake water matrix and no significant matrix effect was found in tap water and lake water except for microcystin-RR. The limits of detection (signal to noise ratio = 3) of toxins were lower than 56.6 ng/L which is far below the 1 μg/L defined by the World Health Organization provisional guideline for microcystin-LR. Finally, this method was successfully applied to lake water samples from Tai lake and proved to be useful for water quality monitoring.
英文摘要: Microcystins and nodularins are cyclic peptide hepatotoxins and tumour promoters from cyanobacteria. The present study describes the development, validation and practical application of a fully automated analytical method based on on-line micro solid-phase extraction-capillary liquid chromatography-tandem mass spectrometry for the simultaneous determination of seven microcystins and nodularin-R in tap water and lake water. Aliquots of just 100 μL of water samples are sufficient for the detection and quantification of all eight toxins. Selected reaction monitoring was used to obtain the highest sensitivity. Good linear calibrations were obtained for microcystins (50–2000 ng/L) and nodularin-R (25–1000 ng/L) in spiked tap water and lake water samples. Excellent interday and intraday repeatability were achieved for eight toxins with relative standard deviation less than 15.7% in three different concentrations. Acceptable recoveries were achieved in the three concentrations with both tap water matrix and lake water matrix and no significant matrix effect was found in tap water and lake water except for microcystin-RR. The limits of detection (signal to noise ratio = 3) of toxins were lower than 56.6 ng/L which is far below the 1 μg/L defined by the World Health Organization provisional guideline for microcystin-LR. Finally, this method was successfully applied to lake water samples from Tai lake and proved to be useful for water quality monitoring.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116055
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Dan YH,Shi XZ,Xu GW. On-Line Micro Solid-Phase Extraction Combined with Capillary Liquid Chromatography-Tandem Mass Spectrometry for High Throughput Analysis of Microcystins and Nodularin-R[C]. 见:36th International Symposium on High Performance Liquid Phase Separations and Related Techniques. 布达佩斯. 2011-6-19.
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