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学科主题: 分析化学
题名: Urinary Metabolic Profiling Analysis to Discover Potential Diagnostic Biomarkers Based on LTQ-Orbitrap High Resolution Mass Spectrometry
作者: Zhou LN(周丽娜) ;  Yin PY(尹沛源) ;  Lu X(路鑫) ;  Xu GW(许国旺)
会议文集: 36th International Symposium on High Performance Liquid Phase Separations and Related Techniques
会议名称: 36th International Symposium on High Performance Liquid Phase Separations and Related Techniques
会议日期: 2011-6-19
出版日期: 2011
会议地点: 布达佩斯
通讯作者: 许国旺
出版者: 待补充
出版地: 待补充
合作性质: 墙报
部门归属: 1808
主办者: Hungarian Society for Separation Sciences
摘要: Urine is a very useful clinical sample and can be collected in a non-invasive form. Information on specific diseases or physiological variables can be taken from the appearance, smell, and taste of urine. Especially, the urinary metabolites are correlated with many levels of metabolic activity. Therefore, in metabolomics, study urine will play a very important role. Unfortunately, because of so many polar metabolites included in urine, its comprehensive analysis is not so easy. In this study, we coupled liquid chromatography to LTQ-Orbitrap MS as a metabolic profiling platform. To get as much metabolite information as possible in a single run, a Waters HSS T3 column was used to increase the retention of polar compounds. Hepatocellular carcinoma (HCC) is known as one of the most common malignancy for its high mortality rate. The exploration of early diagnostic biomarker for HCC is very important for a better surgery outcome. To find the potential early metabolic markers, paired urine samples from the same HCC patient at the diagnosis time and two years before diagnosis (TYBD) were collected. Urine samples from healthy volunteers were used as the control. After peak alignment from metabolic profiling, 550 and 1220 ions, respectively in the positive and negative modes, were imported to SIMCA-P 11.0 for multivariate analysis. Some metabolites were found to be potential early diagnostic biomarkers of HCC. In conclusion, this coupling HSS T3 LC/ LTQ-Orbitrap system is proved effective for metabolic profiling of urine samples and is especially helpful to identify potential biomarkers.
英文摘要: Urine is a very useful clinical sample and can be collected in a non-invasive form. Information on specific diseases or physiological variables can be taken from the appearance, smell, and taste of urine. Especially, the urinary metabolites are correlated with many levels of metabolic activity. Therefore, in metabolomics, study urine will play a very important role. Unfortunately, because of so many polar metabolites included in urine, its comprehensive analysis is not so easy. In this study, we coupled liquid chromatography to LTQ-Orbitrap MS as a metabolic profiling platform. To get as much metabolite information as possible in a single run, a Waters HSS T3 column was used to increase the retention of polar compounds. Hepatocellular carcinoma (HCC) is known as one of the most common malignancy for its high mortality rate. The exploration of early diagnostic biomarker for HCC is very important for a better surgery outcome. To find the potential early metabolic markers, paired urine samples from the same HCC patient at the diagnosis time and two years before diagnosis (TYBD) were collected. Urine samples from healthy volunteers were used as the control. After peak alignment from metabolic profiling, 550 and 1220 ions, respectively in the positive and negative modes, were imported to SIMCA-P 11.0 for multivariate analysis. Some metabolites were found to be potential early diagnostic biomarkers of HCC. In conclusion, this coupling HSS T3 LC/ LTQ-Orbitrap system is proved effective for metabolic profiling of urine samples and is especially helpful to identify potential biomarkers.
内容类型: 会议论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/116057
Appears in Collections:中国科学院大连化学物理研究所_会议论文

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Recommended Citation:
Zhou LN,Yin PY,Lu X,et al. Urinary Metabolic Profiling Analysis to Discover Potential Diagnostic Biomarkers Based on LTQ-Orbitrap High Resolution Mass Spectrometry[C]. 见:36th International Symposium on High Performance Liquid Phase Separations and Related Techniques. 布达佩斯. 2011-6-19.
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