| 学科主题: | 分析化学
|
| 题名: | Improvement of Chromatographic Performances of C8 Hybrid Monoliths by |
| 作者: | Dan YH(单圆鸿)
; Shi XZ(石先哲)
; Dou AB(窦阿波)
; Li H(李华)
; Lu X(路鑫)
; Xu GW(许国旺)
|
| 会议文集: | Proceeding of HPLC 2011
|
| 会议名称: | 37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
|
| 会议日期: | 2011-10-8
|
| 出版日期: | 2011
|
| 会议地点: | 大连
|
| 通讯作者: | 许国旺
|
| 出版者: | 待补充
|
| 出版地: | 待补充
|
| 合作性质: | 墙报
|
| 部门归属: | 1808
|
| 主办者: | 中国化学会色谱专业委员会
|
| 摘要: | Considerable attention has been paid to monolith columns for liquid chromatography
(LC) system owing to their easy preparation, good permeability, fast mass transport
and various kinds of surface modification. The feature of in-situ polymerization make
the monolith column have significant development in capillary/nano LC and
micro-fluidic system research.
In the fabrication of hybrid C8 silica monoliths, the precursor undergoes an acid/base
two-step reaction. Dodecylamine was added to the system after the hydrolysis in
several hours to provide a basic media and also as a template [1]. In this research,
surfactant Pluronic F127 and dodecylamine were used as template together, and the
characteristic of monolith column was explored in detail. The most striking change is
the significant improvement in column efficiency with the addition of F127 in
nano-liquid chromatography mode. When 30 mg F127 was added in the system, the
number of plate was increased almost 2 times of that when no F127 was used. SEM
images show that the size of macropore and skeleton decreased with increasing
amount of F127, which is in accordance with the results observed by Kazuyoshi et al
[2]. With the addition of F127, the column efficiency was increased slightly when the
dodecylamine was reduced to 6mg. The final number of plate was 28,706/meter, with
the minimal plate height of 34.84 μm. Moreover, less amount of dodecylamine delay
the time of sol-gel transition from almost 4 min to more than 10 min, which provide
more time for filling step and improve the column-to-column reproducibility.
The C8 monolith column with 100 μm i.d. was validated in nanoLC-UV system.
Good peak shapes were obtained in the analysis polycyclic aromatic hydrocarbon and
alkylbenzens. The monolith column with 75 μm i.d. was used in nanoLC- nanoESImass
spectrometry to analysis seven kinds of microcystins in water. Without any kind
of preconcentration, the limit of detections of microcystins are all below 1 μg/L given
in the WHO provisional guideline for microcystin-LR in drinking water. |
| 英文摘要: | Considerable attention has been paid to monolith columns for liquid chromatography
(LC) system owing to their easy preparation, good permeability, fast mass transport
and various kinds of surface modification. The feature of in-situ polymerization make
the monolith column have significant development in capillary/nano LC and
micro-fluidic system research.
In the fabrication of hybrid C8 silica monoliths, the precursor undergoes an acid/base
two-step reaction. Dodecylamine was added to the system after the hydrolysis in
several hours to provide a basic media and also as a template [1]. In this research,
surfactant Pluronic F127 and dodecylamine were used as template together, and the
characteristic of monolith column was explored in detail. The most striking change is
the significant improvement in column efficiency with the addition of F127 in
nano-liquid chromatography mode. When 30 mg F127 was added in the system, the
number of plate was increased almost 2 times of that when no F127 was used. SEM
images show that the size of macropore and skeleton decreased with increasing
amount of F127, which is in accordance with the results observed by Kazuyoshi et al
[2]. With the addition of F127, the column efficiency was increased slightly when the
dodecylamine was reduced to 6mg. The final number of plate was 28,706/meter, with
the minimal plate height of 34.84 μm. Moreover, less amount of dodecylamine delay
the time of sol-gel transition from almost 4 min to more than 10 min, which provide
more time for filling step and improve the column-to-column reproducibility.
The C8 monolith column with 100 μm i.d. was validated in nanoLC-UV system.
Good peak shapes were obtained in the analysis polycyclic aromatic hydrocarbon and
alkylbenzens. The monolith column with 75 μm i.d. was used in nanoLC- nanoESImass
spectrometry to analysis seven kinds of microcystins in water. Without any kind
of preconcentration, the limit of detections of microcystins are all below 1 μg/L given
in the WHO provisional guideline for microcystin-LR in drinking water. |
| 内容类型: | 会议论文
|
| URI标识: | http://cas-ir.dicp.ac.cn/handle/321008/116062
|
| Appears in Collections: | 中国科学院大连化学物理研究所_会议论文
|
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| Recommended Citation: | |
Dan YH,Shi XZ,Dou AB,et al. Improvement of Chromatographic Performances of C8 Hybrid Monoliths by[C]. 见:37th International Symposium on High Performance Liquid Phase Separations and Related Techniques. 大连. 2011-10-8.
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