DICP OpenIR
学科主题分析化学
Application of on-line comprehensive two dimensional liquid chromatography
Yang Q(杨芹); Shi XZ(石先哲); Zhao SM(赵素敏); Xu GW(许国旺)
会议文集Proceeding of HPLC 2011
会议名称37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
会议日期2011-10-8
2011
会议地点大连
页码449-0
出版者待补充
出版地待补充
合作性质墙报
部门归属1808
主办者中国化学会色谱专业委员会
英文摘要Triacylglycerides (TAGs) are a large class of complex neutral lipids that naturally occur in both plants and animals. These compounds are important to organisms as they are the main energy resource for biological activities, but when the TAGs were excessively accumulated they could cause metabolic syndromes such as obesity and diabetes. This needs us to better understand the individual TAG molecules or profile their changes in organism and firstly we should isolate these compounds. A direct and effective way to do this is to perform chromatography. However, TAG species are very complex since they are composed by the seterification of a glycerol backbone with three fatty acids, any change of the position or chain length or the number or position of the double bonds of the fatty acids changed the TAG molecule. Thus the separation of TAG puts more demands on the efficiency and peak capacity of the separation approach and an elegant option for this is the comprehensive two dimensional liquid chromatography. In the present work, we used an on-line comprehensive silver-ion liquid chromatography (silver-ion LC) ×reversed-phase liquid chromatography (RP LC) system to analyze triacylglyceride compounds. First dimensional separation was performed on a micro bore silver-ion modified column employing the commonly used hexane-based mobile phase. Optimization was focused on the second dimension, and a series of C18 columns were assessed. A wide bore column packed with 1.5 μm particles was selected as the second dimension column to reduce the negative effect caused by the large volume and strong solvent injection in the second dimension. The system coupled with mass spectrometry was applied to the analysis of the mouse liver extract. Two mouse livers, a yin-deficiency model and its control, were studied and totally 52 TAGs were obtained. The identification was based on the TAGs’ retention behaviors on the comprehensive two-dimensional LC system and their APCI MS fragments. Of the 52 identified TAG, 28 were shared by the two samples, leaving 6 from the model and 18 from the control respectively. This demonstrated that the discrimination between the two types of sample was feasible and so was the application of this separation technique in; Triacylglycerides (TAGs) are a large class of complex neutral lipids that naturally occur in both plants and animals. These compounds are important to organisms as they are the main energy resource for biological activities, but when the TAGs were excessively accumulated they could cause metabolic syndromes such as obesity and diabetes. This needs us to better understand the individual TAG molecules or profile their changes in organism and firstly we should isolate these compounds. A direct and effective way to do this is to perform chromatography. However, TAG species are very complex since they are composed by the seterification of a glycerol backbone with three fatty acids, any change of the position or chain length or the number or position of the double bonds of the fatty acids changed the TAG molecule. Thus the separation of TAG puts more demands on the efficiency and peak capacity of the separation approach and an elegant option for this is the comprehensive two dimensional liquid chromatography. In the present work, we used an on-line comprehensive silver-ion liquid chromatography (silver-ion LC) ×reversed-phase liquid chromatography (RP LC) system to analyze triacylglyceride compounds. First dimensional separation was performed on a micro bore silver-ion modified column employing the commonly used hexane-based mobile phase. Optimization was focused on the second dimension, and a series of C18 columns were assessed. A wide bore column packed with 1.5 μm particles was selected as the second dimension column to reduce the negative effect caused by the large volume and strong solvent injection in the second dimension. The system coupled with mass spectrometry was applied to the analysis of the mouse liver extract. Two mouse livers, a yin-deficiency model and its control, were studied and totally 52 TAGs were obtained. The identification was based on the TAGs’ retention behaviors on the comprehensive two-dimensional LC system and their APCI MS fragments. Of the 52 identified TAG, 28 were shared by the two samples, leaving 6 from the model and 18 from the control respectively. This demonstrated that the discrimination between the two types of sample was feasible and so was the application of this separation technique in
文献类型会议论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/116063
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺)
推荐引用方式
GB/T 7714
Yang Q,Shi XZ,Zhao SM,et al. Application of on-line comprehensive two dimensional liquid chromatography[C]. 待补充:待补充,2011:449-0.
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