DICP OpenIR
Subject Area分析化学
Development of off-line two dimensional liquid chromatography-mass
Zhao SM(赵素敏); Yang Q(杨芹); Shi XZ(石先哲); Lu X(路鑫); Xu GW(许国旺)
Source PublicationProceeding of HPLC 2011
Conference Name37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
Conference Date2011-10-8
2011
Conference Place大连
Pages451-0
Publisher待补充
Publication Place待补充
Cooperation Status墙报
Department1808
Funding Organization中国化学会色谱专业委员会
AbstractLipidomics is a rising field and has been concerned by more and more researchers, due to lipid metabolism is one of the three metabolism of human being, and various influencing human health diseases all have a relationship with lipids, such as cancer, diabetes and heart disease. Liquid chromatography is a powerful tool for the separation and analysis of lipids. Although the separation technique is developed constantly, one dimensional chromatography still has not sufficient power to separate the lipids in comprehensive biological samples adequately, neither a reverse phase chromatography, nor a normal phase chromatography. In our study, we developed an off-line two dimensional chromatography method for separation and analysis of lipids from comprehensive biological samples. A diol normal phase column was employed as the first dimension, and a T3 reverse phase column was used as the second dimension. The retention time of the first dimension was stabilized by suitable equilibrium condition, so the lipid fractions were enriched and much more lipids of liver have been identified by the off-line two dimensional systems than that by only the first dimension.; Lipidomics is a rising field and has been concerned by more and more researchers, due to lipid metabolism is one of the three metabolism of human being, and various influencing human health diseases all have a relationship with lipids, such as cancer, diabetes and heart disease. Liquid chromatography is a powerful tool for the separation and analysis of lipids. Although the separation technique is developed constantly, one dimensional chromatography still has not sufficient power to separate the lipids in comprehensive biological samples adequately, neither a reverse phase chromatography, nor a normal phase chromatography. In our study, we developed an off-line two dimensional chromatography method for separation and analysis of lipids from comprehensive biological samples. A diol normal phase column was employed as the first dimension, and a T3 reverse phase column was used as the second dimension. The retention time of the first dimension was stabilized by suitable equilibrium condition, so the lipid fractions were enriched and much more lipids of liver have been identified by the off-line two dimensional systems than that by only the first dimension.
Document Type会议论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/116064
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
Recommended Citation
GB/T 7714
Zhao SM,Yang Q,Shi XZ,et al. Development of off-line two dimensional liquid chromatography-mass[C]. 待补充:待补充,2011:451-0.
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